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تم العثور علي : 6
 تم العثور علي : 6
  
 
إعادة البحث

Thesis 2017.
Awad, Basma Mosilhy Kassem.
615 علم الصيدلة والعلاج  
pharmacy - Abstract:
Historically - plants have been used since ancient times and in folklore for the treatment of many diseases and illnesses including chronic diseases and cancer care. In recent years - plants have been getting more attention as drugs worldwide.
Achillea fragrantissima (Forssk.) Sch. Bip. is a desert plant which belongs to Asteraceae family and is known as Qaysūm in Arabic. We selected it as a member of family Asteraceae to be the plant under investigation. It grows in Sinai - Egypt and is used in folk medicine by Bedouins for treatment of many diseases. In addition it has been tested for its anticancer activity. The broad aim of this thesis was to conduct a phytochemical investigation and biological confirmation of the plant Achillea fragrantissima (Forssk.) Sch. Bip. and its constituents as an anticancer agents illustrating their mechanism of action.
The initial part of this work focused on large scale extraction of the plant with different solvents - bioactivity-guided fractionation led to the isolation of seven compounds followed by determination of the chemical nature of them. The isolated compounds are:
The known compounds Eupatilin 7- methyl ether (3) - chrysosplenol D (4) - Cirsiliol (5) - β-Sitosterol-3-O-β-D-glucoside (6) and Cirsimaritin (7) while Piceol (1) and Veratric acid (2) are reported here for the first time from Achillea fragrantissima (Forssk.) Sch. Bip.
The second part of this work focused on evaluation of the in-vitro cytotoxic activity of the methanolic extract of A. fragrantissima (Forssk.) Sch. Bip - its fractions and the isolated compounds 1 - 2 - 3 - 4 - 5 and 7 against different cell lines using the Sulpho-Rhodamine-B (SRB) assay. The extract - fractions (4 - 5) and the compounds 3 - 4 - 5 and 7 showed high cytotoxic activities when compared to appropriate reference drug (doxorubicin) and IC50 values (the concentrations of drug required to produce 50% inhibition of cell growth) were calculated for the isolated compounds.
The third part of this work focused on evaluation of the in-vivo antitumor activity of the methanolic extract of A. fragrantissima (Forssk.) Sch. Bip. and its isolated compounds 3 - 4 - 5 and 7 using Ehrlich’s Ascites Carcinoma (EAC) model in mice. A. fragrantissima (Forssk.) Sch. Bip. extract and its isolated compounds reduced the tumor weight compared to EAC-Control group. In addition - the compounds 3 - 7 treatments produced a dose dependent reduction in tumor weight. Determination of serum tumor necrosis factor – alpha (TNF-α) level highlighted that A. fragrantissima (Forssk.) Sch. Bip. extract and its isolated compounds significantly reduced its serum level compared to the EAC-Control group - where 3 - 5 - 7 groups produced a significant reduction in serum level of TNF-α compared to all other groups. Furthermore - A. fragrantissima (Forssk.) Sch. Bip. extract and the isolated compounds significantly increased the total antioxidant capacity (TAC) compared to EAC-Control especially compound 7. The histopathological examination revealed that A. fragrantissima (Forssk.) Sch. Bip. extract and its isolated compounds produced a better differentiation of tumor cells - with reduced nuclear pleomorphism and better formed tubular structures especially with high dose indicating the induction of apoptotic mechanism.
Finally - an in silico study was performed as a step to elucidate the mechanism of the cytotoxic effect of the compounds isolated from A. fragrantissima (Forssk.) Sch. Bip - where the structure of cirsiliol (5) was docked into the active sites of the crystal structures of PI3K and Akt - two protein kinases that are significantly involved in prostate and breast cancer processes.
Key Words: Achillea fragrantissima (Forssk.) Sch. Bip - anticancer - Ehrlich’s Ascites Carcinoma - Docking.
Aim of work:
Flora of Egypt especially in Sinai Peninsula is rich and diverse with medicinal and aromatic plants as well as herbs and spices. These plants have been used by local people in folk medicine for curing human and animal illnesses. The plants of genus Achillea as a part of Sinai Peninsula flora were and still a rich source of valuable natural products for treatment of many diseases; therefore - this study was designed to fulfill:
1. Collection of the plant material from Sinai Peninsula in Egypt.
2. Bioactivity-guided fractionation to isolate active compounds using different chromatographic techniques.
3. Identification and structure elucidation of the isolated compounds - using 1D NMR - 2D NMR and DEPT measurements.
4. In vitro cytotoxic and in vivo antitumor evaluations of the methanolic extract of Achillea fragrantissima (Forssk.) Sch. Bip. and its isolated compounds.
pharmacognosy
تصفح الوعاء  

Book 1935.
Breasted, James Henry.
930 تاريخ العالم القديم   930 تاريخ العالم القديم  
History of Early World - Ancient times - History
Ginn and company,

Book 1992 .
O’neill, Amanda.
ISBN: 1856273741
709 المعالجة التاريخية والجغرافية ومعالجة الأشخاص  
ancient times
grandbook,

Book [-198].
Breasted, James Henry.
932 تاريخ مصر  
Ancient Times
Ginn And Company,

Book 1959.
Swain, Joseph Ward.
909 تاريخ العالم  
History - History
Harper & brothers publishers,

Book 1959 .
Swain, Joseph Ward.
909 تاريخ العالم  
History - History
Harper - brothers publishers,

 







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