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[9000436.] رقم البحث : 9000436 - |
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EFFECT OF L-CARNITINE ON PILOCARPINEINDUCED / |
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قطاع الدراسات الطبية / الصيدليه السريريه |
| تخصص البحث : الصيدليه السريريه |
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Menoufia Medical Journal / / Vol. 25, No. 1 - Janury 2012 |
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ط/ محمد فاروق احمد سيد احمد |
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hippocampus - l-carnitine - oxidative stress - pilocarpine - epilepsy |
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Reactive oxygen species have been implicated in seizure-induced
neurodegeneration and there is a correlation between free radical level
and scavenger enzymatic activity in epilepsy. It has been suggested that
pilocarpine-induced seizures is mediated by an increase in oxidative
stress. Current research has found that antioxidant may provide, in a
certain degree, neuroprotection against the neurotoxicity of seizures at
the cellular level. L-Carnitine has a powerful antioxidant action. The
objective of the present study was to evaluate the neuroprotective effects
of L-Carnitine (L-CAR) in rats, against oxidative stress caused by
pilocarpine-induced seizures. 30 min prior to behavioral observation, rats
were treated with (0.9% saline i.p., control group), L-CAR 300 mg/k
alone (L-CAR 300 mg/kg i.p., L-CAR group), pilocarpine (400 mg/kg, i.p.,
P400 group) and the combination of L-CAR (300 mg/kg, i.p.) and
pilocarpine (400 mg/kg, i.p.). After the treatments all groups were
observed for 6 hr. The enzymatic activities, lipid peroxidation and nitrite
concentrations were measured using speccitrophotometric methods and
these data were assayed. In P400 group rat there was a significant
increase in lipid peroxidation and nitrite levels. However, no alteration
was observed in superoxide dismutase (SOD) and catalase activities. In
the L-CAR and pilocarpine co-administered rat, antioxidant treatment
significantly reduced the lipid peroxidation level and nitrite content, as
well as increased the SOD and catalase activities in rat hippocampus
after seizures. Our findings strongly support the hypothesis that oxidative
stress occurs in hippocampus during pilocarpine-induced seizures,
indicate that brain damage induced by the oxidative process plays a
crucial role in seizures pathogenic consequences, and imply that strong
protective effect could be achieved using l-carnitine. In conclusion: Lcarnitine
could enhance activities of SOD and reduce the MDA level and
could reduce seizure period, inhibit neuronal damage as free radical
scavenger. |
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