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[9000441.] رقم البحث : 9000441 - |
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HISTOLOGICAL, IMMUNOHISTOCHEMICAL AND BIOCHEMICAL / |
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قطاع الدراسات الطبية / الصيدليه السريريه |
| تخصص البحث : الصيدليه السريريه |
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Menoufia Medical Journal / / Vol. 25, No. 2 - July 2012 |
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محمـد أحمد عبد الفتاح عطية المهدى |
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Clinical Pharmacology, Biochemistry and Histology |
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Background: Diabetes mellitus and its complication have reached epidemic proportions
worldwide. Exenatide is one of glucagon -like peptide-1 (GLP-1) receptor agonists. It is
the synthetically derived peptide of exendin-4. It has been shown to improve glycemic
state in patients with type 2 diabetes. The aim of the work was to study the effect of
exenatide on islets of Langerhans of diabetic adult male rats based on histological,
immunohistochemical and biochemical study.Materials and Methods: 30 adult male
albino rats were divided into four groups; the 1st group was –ve control, the 2nd group was
given exenatide at a dose of 2 ,g/ kg twice daily by S.C route and served as +ve control.
The 3rd group was given streptozotocin (STZ) as a single dose of 60mg/ kg I.P to induce
diabetes. The 4th group was given exenatide, 4 days after administration of STZ. Blood
glucose level was estimated daily and after one week, serum glucagon- like peptide
(GLP-1) and insulin levels were also measured. Sections of pancreas were prepared and
stained with H&E, Masson trichrome and immunohistochemical staining of B- cells by
anti-insulin antibody. The density (area %) of anti-insulin antibody reaction and number of
B- cells were studied. Results of diabetic group (GIII) showed marked degenerative and
apoptotic changes of cells mostly in the centers of islets with insulinitis. Also deposition of
connective tissue around and in between islets cells was seen. Moreover, there was
reduction in area % of anti-insulin antibody reaction and number of B- cells. Biochemical
results showed elevation of blood glucose level, reduction of insulin level and non
significant change of GLP-1 level compared to control group (G1). Administration of
exenatide resulted in improvement of morphological picture of islets cells mainly of Bcells.
Moreover, the previously mentioned morphometric parameters detected significant
increase. There was lowering of blood glucose level, elevation of insulin level and non
significant change of GLP-1 Level. Conclusion: It is concluded that exenatide is effective
drug in reducing the degenerative changes of B- cells associated with diabetes mellitus.
This could be explained by the agonistic activity of exenatide on GLP-1 receptor
producing GLP-1 mimetic effects. Such effect makes this drug a beneficial drug to be
used in diabetic patients.
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