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[9004575.] رقم البحث : 9004575 - |
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VIRULENCE OF ISOLATED PSEUDOMONAS AERUGINOSA INFECTING DUCKLING AND ANTIBIOTIC RESISTANCE WITH AN EXPERIMENTAL TREATMENT TRIAL / الضراوة لبكتريا السودوموناس ايرجينوزا المعزولة من البط الصغير ومقاومته للمضادات الحيوية مع محاوله تجريبيه للعلاج |
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قطاع الدراسات البيطرية / General |
| تخصص البحث : General |
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Assiut veterinary medical journal / المجلد 67 العدد 169 - ابريل 2021 |
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اسماء رفعت احمد ابراهيم |
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مصطفى صفوت عبده ( dr.mostafaabdou@yahoo.com - ) - مؤلف رئيسي |
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عاطف عبدالمجيد سالم موسى |
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محمد فهمى الدكرورى |
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Pseudomonas aeruginosa, duckling, Virulence genes, antibiotic resistance genes, sensitivity test, Experimental infection. |
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The present study was conducted in Kafrelsheikh governorate to isolate P.aeruginosa from 120 ducks, sampling from cloacal swab, internal organs and tracheal swab of freshly dead, healthy and diseased ducklings at different ages which showed depression, emaciation, ocular and or nasal discharges, diarrhea and sometimes enlargement of hock joint and had lesions of airsacculitis, congested liver, catarrhal enteritis and sometimes presence of gelatinous material in the hock joints with rate of isolation of 16.66% (20 samples were positive). Serological identification of suspected isolates of P. aeruginosa showed that 16 isolates of polyvalent I group I and 4 isolates of polyvalent II group J. Susceptibility of the isolated P. aeruginosa for 11 antibiotics demonstrated that the highest resistance was noted against Erythromycin, Oxytetracycline, Ampicillin and Amoxicillin (95%). The highest sensitivity of P. aeruginosa was observed against Florphenicol (80%) and it used for treatment in experimental design and give good results. Multiplex PCR used to identify virulence represented by toxA gene, lasI gene and oprL gene of P. aeruginosa show that all isolates where positive for the 3 examined virulence genes and these indicate that all isolates were highly virulent strains and antibiotic resistance genes represented by qnrS gene, blaCTX gene and mexR gene of P. aeruginosa showed that all isolates where positive for the 3 examined AB resistance exept isolate No 4 were negative for blaCTX and these indicate that all isolates were highly AB resistance exept isolate No 4 was not have blaCTX resistance gene. Experimental infection was done to study the Pathogenicity of P. aeruginosa which isolated from diseased ducklings on newly hatched ducklings with dose of infection of 0.2ml of 1x107 cfu/ml s/c and use the potent antibiotic florphenicol for treatment of experimentally infected ducks. Our study proved that virulence genes owned by the P. aeruginosa confirming its pathogenicity for ducks, especially in the presence of oprL gene which plays a great role in antimicrobial resistance, so biosafety was recommended for hatcheries and farms, the hygiene, cleaning and disinfection will reduce P. aeruginosa spreading in the farms. |
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