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[9002246.] رقم البحث : 9002246 - |
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A Study on the factors affecting biomass formation by a highly kojic acid producer fungal isolate from sugarcane molasses / دراسات علي العوامل المؤثرة علي انتاج الكتلة الحيوية بواسطة الفطريات المنتجة لأعلي كمية من حمض الكوجيك المنتج من قصب السكر المصري |
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قطاع الدراسات الزراعية / |
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Egyptian sٍugar journal / / vol.11 - 19/12/2018 |
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اسماء فتحى محمود - مؤلف رئيسي |
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Abdel-Naser A. Zohri |
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Ghada Abd-Elmonsef Mahmoud |
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Nermien H. Sadik |
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Radwa Adel Hanafy |
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sugarcane molasses
producer fungal isolate |
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Nowadays microbial production of natural products represent a hot spot point in our environment and become an alternative way to chemical synthetic. In our research Aspergillus flavus No. 3 shows a great power in both biomass and kojic acid production. Plackett–Burman design utilize Egyptian sugarcane molasses as a sole carbon source giving kojic acid production of 0.82 (predicted 1.04) g/l to 24.65 (predicted 23.74) g/l, consuming sugar at 27.33 (predicted 26.96) % to 89.87 (predicted 87.46) % and forming dry biomass between 3.6 (predicted 3.8) g/l and 28.2 (predicted 28.05) g/l. The maximum kojic acid (24.65 g/l) and biomass value (28.2 g/l) obtained at 25°C; 9, 5 days of incubation, pH 3, 5; 0.5%, 2% inoculum size and shaking rate at 150 rpm using fermentation medium (g/l) of sugarcane molasses, 60; yeast extract, 7, 3; KH2PO4, 2; ZnSO4.7H2O, 0.001 and MgSO4.7H2O, 1, respectively.
Nowadays microbial production of natural products represent a hot spot point in our environment and become an alternative way to chemical synthetic. In our research Aspergillus flavus No. 3 shows a great power in both biomass and kojic acid production. Plackett–Burman design utilize Egyptian sugarcane molasses as a sole carbon source giving kojic acid production of 0.82 (predicted 1.04) g/l to 24.65 (predicted 23.74) g/l, consuming sugar at 27.33 (predicted 26.96) % to 89.87 (predicted 87.46) % and forming dry biomass between 3.6 (predicted 3.8) g/l and 28.2 (predicted 28.05) g/l. The maximum kojic acid (24.65 g/l) and biomass value (28.2 g/l) obtained at 25°C; 9, 5 days of incubation, pH 3, 5; 0.5%, 2% inoculum size and shaking rate at 150 rpm using fermentation medium (g/l) of sugarcane molasses, 60; yeast extract, 7, 3; KH2PO4, 2; ZnSO4.7H2O, 0.001 and MgSO4.7H2O, 1, respectively |
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