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Egyptian sٍugar journal /
 Egyptian sٍugar journal /
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[9001097.] رقم البحث : 9001097 -
Assessment of Somaclonal Variation, Correlation and Stepwise Regression to Evaluate new Sugarcane Somaclones /
  قطاع الدراسات الزراعية /
تخصص البحث :
  Egyptian sٍugar journal / / vol.8 - 20/6/2016
  اسماء فتحى محمود - مؤلف رئيسي
  Tissue culture
Sugar crop
Sterwise regression
  Significant differences could be found among the somaclones each other and their donor GT-54 9 for all studied traits in plant cane as well as first and second ratoon crops. Moreover, the highest values of genotypic (GCV) and phenotypic variations (PCV) were correlated with reducing sugar in all three successive seasons recording 20.55 and 22.67, 8.27 and 8.93, and 10.53 and 10.73% in plant cane, first and second ratoon crops, respectively. The obtained significant differences coupled with GCV, PCV and heritability estimates explained the differences among the studied genotypes regenerated from the immature leaves of GT- 54 9 variety of sugarcane which named somaclonal variation. The somaclone no. 6 surpassed significantly the donor GT 54-9 for purity % and sugar yield in plant cane and first ratoon crops and insignificant in second ratoon crops. High and positive estimates of correlation coefficients were recorded between sugar yield and each of sugar % in gram, sweetness % and purity % in plant cane and both first and second ratoon crops, which accounted 0.851, 0.843 and 0.861 in plant cane, 0.861, 0.911 and 0.624 in first and 0.973, 0.987 and 0.903 in second ratoon crops, respectively. Moreover, sugar yield correlated in high positive values with brix volumetric in first (0.682) and second (0.951) ratoon crops, as well as with brix weighted (0.935) in second ratoon crop. Also, the weight of millable stalk/plot possessed high and positive estimates of correlation with number of millable stalk/plot across the three respective crops (0.604 ? 0.900). It is remark result that the traits of brix volumetric, brix weighted, sugar% in gram, sweetness %, purity % and sugar yield were correlated each other in high and positive estimates in range of 0.795 to 0.996 in second ratoon crop. It is clear result revealed from stepwise regression that the first model includes only sweetness % (as in the first ratoon), but with high R2 of 0.972. Moreover, the fitted model no. 2 includes sweetness % and purity % with R2 equal to unity (=1). Consequently, this model is superior one and could be used to determine those two traits for selection to high sugar yield in sugarcane. The expected sugar yields for all fitted stepwise models were insignificant different relative to the actual sugar yield in the three successive crops as revealed by t-test which tend to be zero in some models. Moreover, the correlation coefficients between expected and actual sugar yield were positive, very high and reached to the unity in second ratoon crop. These results displayed the effeteness of stepwise regression analysis to determine the strongest trait/s to in contribution to high sugar yield even from cane and quality components in sugarcane.
Key words: sugarcane, Immature leaves, callus, somaclones, somaclonal variation, correlation, stepwise regression.
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