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[9000496.] رقم البحث : 9000496 - |
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Toxicity of Heavy Metals to Agrotis ipsilon (Hufn.) and the Entomopathogenic Nematode, Steinernema carpocapsae / |
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Agricultural Research Journal Suez Canal University / / Volume 10, No. 1 - 2010 |
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M. M. A. El-shershaby |
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Agrotis ipsilon; mortality; pupation percentage; heavy metals; instar larvae; pupal weight; emerged adults. |
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Three different concentrations (A,B&C) of some heavy metals (Ni, Zn, Mo, Cu, & Pb) were evaluated by contact using treated and sterile sand against 4th instar larvae of Agrotis ipsilon (Hufn) under laboratory conditions. Larval mortality, pupation percentage, pupal weight, percentage of emerged adults from formed pupae and malformation percentage of emerged adults were calculated. Results obtained that, larval mortality was ranged between 28% at high tested concentration of Ni and 4% at lowest tested concentration of Cu and Zn, also at control. Pupation percentage was 96% at treatment with concentrations A, B of Pb and Concentration C of Zn and Cu also at control. Treatment with Ni recorded 100% and 72% pupation at concentrations C and A, respectively. Pupal weight was ranged between 0.31 and 0.37gm at treatments while it was 0.41gm at control. Effect on adult emergence was very clear especially at Zn and Pb treatments which obtained no emergence while the slight effect was observed at Ni and Mo treatments (70% adult emergence). Malformation percentage of emerged adults reached 50% at concentrations C, B of Zn decreased to 10% at three tested concentrations of Copper. Toxicity heavy metals to the entomopathogenic nematode Steinernema carpocapsae was also studied and results obtained that, maximum nematodes (IJs) mortality was 71.1% at concentration A of Ni and the minimum was 23.1% at treatment with concentration C of Mo, while it was 2.9% at control. Virulence of treated juveniles for 48h to 4th instar A. ipsilon was clearly affected, it was ranged between 19.4% and 74.2% at concentration A of Zn and concentration C of Mo, respectively, while control recorded 94.4% successful pathogencity. |
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