EFFECT OF ESSENTIAL OILS ON TRANSDERMAL PERMEATION OF METOPROLOL SUCCINATE / تأثير الزيوت الأساسية على نفاذية سكسينات الميتوبرولول عبر الجلد
قطاع الدراسات الصيدلية / الصيدلانيات
تخصص البحث : الصيدلانيات
Bulletin of Pharmaceutical sciences / / vol.44 الجزء الأول - 1/7/2021
تاريخ تقديم البحث 19/08/2021
تاريخ قبول البحث 26/08/2021
عدد صفحات البحث 14
سارة يوسف محمد( Sarahyousef30@yahoo.com - )
د/ ميهول باتيل - مؤلف رئيسي
الصيدلانيات
The objective of the present study was to prepare a matrix-type transdermal drug delivery system containing metoprolol succinate and to evaluate the effect of essential oils on transdermal permeation of drug. The solvent evaporation method was employed to fabricate transdermal patches of drug using HPMC K15M and Acrycoat L100 as polymers and polyethylene glycol 400 as a plasticizer. Prepared patches were subjected for evaluation of physical as well as physicochemical properties, moisture characteristics, and ex-vivo drug permeation studies. The results of physical characterization showed uniform casting and good appearance of patches. Satisfactory mechanical strength was revealed in the results of tensile strength and folding endurance. Ex-vivo permeation study indicated enhancing effect of essential oils on drug permeation through the skin. The maximum flux observed was 94 ?g/cm2.hr achieved by formulation M3 containing lemongrass oil (20%). The results suggested that lemongrass oil was effective at higher concentration (20%) after 14 hrs of permeation while peppermint oil and eucalyptus oil were effective at low concentration (10%). Release kinetic suggested diffusion-controlled release of drug which followed the Korsmeyer-Peppas model. In conclusion, essential oils can be utilized as a safe and effective alternative for the permeation enhancement of drugs through transdermal delivery.
FORMULATION DEVELOPMENT AND EVALUATION OF HIGHLY WATER-SOLUBLE DRUG-LOADED CONTROLLED RELEASE MATRIX TABLETS / تطوير الصياغة وتقييم أقراص المصفوفة محملة بالعقار منضبطة الانطلاق وعالية الذوبان في الماء
قطاع الدراسات الصيدلية / الصيدلانيات
تخصص البحث : الصيدلانيات
Bulletin of Pharmaceutical sciences / / vol.44 الجزء الأول - 1/7/2021
تاريخ تقديم البحث 19/08/2021
تاريخ قبول البحث 26/08/2021
عدد صفحات البحث 15
سارة يوسف محمد( Sarahyousef30@yahoo.com - )
د/ محمد إقبال ناصرى - مؤلف رئيسي
الصيدلانيات
Formulation of controlled release matrix tablets of Itopride hydrochloride (ITP) was done using direct compression method. Different polymers were used to evaluate the influence of different types (HPM, EC, Kollidon® SR), concentration (20-40%) and viscosity grade (HPMC-4000 cps, HPMC-100000 cps, EC-10 cps and Kollidon SR) on drug release. Twelve different tablet formulations were designed with constant amount of ITP in each tablet formulation (150 mg). The dissolution studies of CR matrix formulations were carried out in acidic buffer (pH 1.2) and phosphate buffer (pH 6.8). Drug release kinetics was studied for first order, Zero-order, Higuchi, Korsmeyer-Peppas and Weibull models using DD Solver (an add-in software for MS Excel). Formulation ECF7, ECF8 and ECF9 containing EC (20-40%) greatly controlled the release rate of drug over an extended period of 12 hr. However, drug release from tablets formulations K4F3, K100F5 and ECF8, followed zero-order kinetics with regression coefficient of 0.966-0.999. The release mechanism of tablets formulations K4F2, KK4F3, K4F4, ECF7, KSRF10, KSRF11 and KSRF12 were non-fickian diffusion, whereas the release mechanism from formulations K4F1, K100F5, K100F6, ECF8 and ECF9 were super case-II transport mechanism. It was concluded that HPMC and ethylcellulose (EC-10cps) in the percentage range of 20-30% were excellent release controlling polymers for itopride HCl.
PHARMACOKINETIC SIMULATION AND OPTIMIZING GENTAMICIN DOSING IN PEDIATRICS / محاكاة الحركة الدوائية للجنتاميسن في الأطفال واقتراح نظام الجرعات الامثل
قطاع الدراسات الصيدلية / الصيدلانيات
تخصص البحث : الصيدلانيات
Bulletin of Pharmaceutical sciences / / vol.44 الجزء الأول - 1/7/2021
تاريخ تقديم البحث 19/08/2021
تاريخ قبول البحث 26/08/2021
عدد صفحات البحث 9
سارة يوسف محمد( Sarahyousef30@yahoo.com - )
د/ أحمد شاكر على - مؤلف رئيسي
الصيدلانيات
Aim: This study was conducted to optimize gentamicin (genta) use in paediatrics incorporating the estimation of serum drug concentrations and customized pharmacokinetic (PK) simulations. Method: 66 patients (age 1-144 month) were enrolled in the study. They received gentamicin 2-2.5 mg/kg every 8 hr. (TID regimen). Serum genta concentrations within one dosing interval of dose 3 and dose 4 were determined using an immunoassay; blood samples were collected at 30 minutes after the end of infusion while a trough level sample was collected just before the next dose. Customized PK simulation analysis was based on the following assumptions: Single-compartment model, first-order elimination, and repeated short time IV infusion. PK parameters and simulation of genta peak/trough levels after various regimens were estimated and compared statistically. Results: About 65% of the patients showed subtherapeutic peak genta levels (less than 6 ug/ml) during the dosing intervals. Potentially toxic trough levels (>1 ug/ml) were observed in two patients. Neonates (1-12 months) showed a relatively higher mean genta volume of distribution (Vd), 0.51±0.18 L/Kg, vs 0.37±0.13 (p<0.05) in children (>1-12 y). Half-life in both groups was comparable (about 3 h). Simulation suggests BID regimens will provide the best theoretically overall peak/trough targets. Discussion: In children, a higher volume of distribution of genta could be associated with the lower serum peak levels due to the conventional dosing regimen (TID). Conclusion: Optimal dosing regimen in pediatric patients can be designed to achieve target high peak, low trough levels based on simplified customized PK simulations.
ANTI-HYPERGLYCEMIC ACTIVITY OF TOTAL ETHANOLIC FRUIT EXTRACT OF FAIDHERBIA ALBIDA ON NICOTINAMIDE-STREPTOZOTOCIN-INDUCED DIABETIC MICE / التأثير المضاد لفرط سكر الدم لخلاصة الإيثانول الكلية لثمرات نبات فايدهربيا البيدا في الفئران المصابة بداء السكري التي يسببها النيكوتيناميد والستربتوزوتوسين
قطاع الدراسات الصيدلية / العقاقير
تخصص البحث : العقاقير
Bulletin of Pharmaceutical sciences / / vol.44 الجزء الأول - 1/7/2021
تاريخ تقديم البحث 19/08/2021
تاريخ قبول البحث 26/08/2021
عدد صفحات البحث 8
سارة يوسف محمد( Sarahyousef30@yahoo.com - )
د/ لورين جمال جبرائيل - مؤلف رئيسي
العقاقير
Faidherbia albida is one of the plants that have been traditionally used throughout the world in the treatment of diabetes. In previous studies, various parts of the plant such as the methanolic root bark, aqueous seed, and the aqueous stem bark extracts have been tested on alloxan-induced diabetic rats for their anti-hyperglycemic activity. In the current study, the anti-hyperglycemic activity of the total ethanolic extract of Faidherbia albida fruits was evaluated in nicotinamide-streptozotocin-induced diabetic mice using glimepiride as a reference anti-diabetic drug. The total ethanolic fruit extract at 200 mg/kg body weight significantly (p< 0.05) lowered the blood glucose level in diabetic mice by (74%) after 4 hrs of oral administration while the peak hypoglycemic effect of glimepiride (55.2%) occurred at the 4th hr after oral administration. The results provided evidence that Faidherbia albida fruit extract is recommended to be used as a hypoglycemic drug in treating diabetic patients.
PROXIMATE AND ELEMENTAL ANALYSES, PHYTOCHEMICAL SCREENING AND ANTIOXIDANT ACTIVITIES OF AQUEOUS AND ETHANOL EXTRACTS OF SOLANUM INCANUM LINN. FRUITS / التحليلات التقريبية والتحليلات للعناصر ، والفحص الكيميائي النباتي وأنشطة مضادات الأكسدة للمستخلصات المائية والإيثانولية لثمار نبات الحدق الغباري
قطاع الدراسات الصيدلية / العقاقير
تخصص البحث : العقاقير
Bulletin of Pharmaceutical sciences / / vol.44 الجزء الأول - 1/7/2021
تاريخ تقديم البحث 19/08/2021
تاريخ قبول البحث 26/08/2021
عدد صفحات البحث 14
سارة يوسف محمد( Sarahyousef30@yahoo.com - )
د/ أ. أو. أكانمو - مؤلف رئيسي
العقاقير
Solanum incanum Linn. (Solanaceae) is a perennial bushy herb used to stained teeth among the Kanuri women and in making vegetable soups. This study determined proximate and elemental contents, phytochemical constituents and antioxidant activities of the fruit. The proximate analyses showed the presence of crude fibre, carbonhydrate and crude protein while the elemental analyses revealed the presence of magnesium, calcium and sodium. The fruit contains; saponins, tannins, flavonoids, terpenoids, cardenolite, glycosides, reducing sugars, phenolics content and flavonoids content. The results of antioxidant showed that the EC50 values for DPPH radicals with aqueous and ethanol fruit extracts of the S. incanum Linn. were found to be 0.02488 and 0.1000 mg/ml, respectively. The aqueous extract showed EC50 value lower than ethanol extract. In conclusion, this results suggested that aqueous extract of S. incanum Linn might contain more potential antioxidant compounds than ethanol extract.
IN-VITRO ANTIBACTERIAL ACTIVITY AND CHEMICAL COMPOSITION OF SYRIAN ERICA MANIPULIFLORA ESSENTIAL OIL / الفعالية المضادة للبكتريا في الزجاج والتركيب الكيميائي للزيت العطري لنبات العجرم (Erica manipuliflora) السوري
قطاع الدراسات الصيدلية / العقاقير
تخصص البحث : العقاقير
Bulletin of Pharmaceutical sciences / / vol.44 الجزء الأول - 1/7/2021
تاريخ تقديم البحث 19/08/2021
تاريخ قبول البحث 26/08/2021
عدد صفحات البحث 6
سارة يوسف محمد( Sarahyousef30@yahoo.com - )
د/ لميس طلال طلاس - مؤلف رئيسي
العقاقير
In current research, we aimed to investigate the chemical composition of the EO of Erica manipuliflora extracted in two growth periods (before flowering and full flowering periods), to evaluate the differences between the two EOs in antibacterial activity against four pathogenic bacteria (Bacillus subtilis, Staphylococcus aureus, Escherichia coli and Salmonella enteritidis). Results showed the existence of 17 and 23 volatile components in the EOs extracted from E. manipuliflora before flowering and in full flowering periods, respectively. The main components in EO extracted from the plant before flowering period were Germacrene-D, tau-cadinol, ?-caryophellene, Trans-pinocarveol and ?-pinene; which represented 35.02, 26.36, 14.78, 9.25 and 3.65%, respectively. The EO extracted from the plant collected in full flowering period were ?-citral, ?-pinene, Germacrene-D, tau-cadinol and Trans-pinocarveol, which represented 22.26, 18.50, 16.90, 11.89 and 5.58%, respectively. The EO extracted from E. manipuliflora in the full flowering period was more active than that extracted before flowering period towards the bacterial species mentioned above. The most sensitive bacterium towards E. manipuliflora EOs was Bacillus subtilis, while the most resistant one was Salmonella enteritidis.
IN-SILICO AND IN-VITRO BACTERICIDAL ACTIVITY OF THE PHYTOCHEMICALS OF PEPEROMIA PELLUCIDA (L.) HERB / المواد الكيميائية النباتية لعشب البيبروميا بيلوسيدا كمضاد الجراثيم حاسوبيا وفي المختبر خارج الخلية
قطاع الدراسات الصيدلية / العقاقير
تخصص البحث : العقاقير
Bulletin of Pharmaceutical sciences / / vol.44 الجزء الأول - 1/7/2021
تاريخ تقديم البحث 19/08/2021
تاريخ قبول البحث 26/08/2021
عدد صفحات البحث 8
سارة يوسف محمد( Sarahyousef30@yahoo.com - )
د/ مودوكريشنايا كوتاكوندا - مؤلف رئيسي
العقاقير
Background: The development and spread of pathogenic antibiotic resistance to many antibiotics have been growing worldwide. This concept is of enormous importance, and it is crucial to identify appropriate therapeutic drugs to fight against these antibiotic-resistant microbes. Plant species can make a valuable contribution to new antibiotics to replace or improve existing treatment potential. Methods: In this work, we conducted a molecular modelling study of three phytochemicals (Peperomin A, Peperomin E, Peperomin F) with bacterial targets penicillin-binding protein (2C6W) from Streptococcus pneumoniae and kdpFABC complex (5MRW) of Escherichia coli. Peperomia pellucida methanolic extract was conducted antimicrobial activity against clinical Streptococcus pneumoniae and Escherichia coli. Results: Three phytochemicals (Peperomin A, Peperomin E, Peperomin F) showed the best docking results with target antimicrobial-resistant targets. Peperomia pellucida methanolic extract showed good antibacterial activity against clinical Streptococcus pneumoniae and Escherichia coli. Conclusion: In-vitro and In silico bactericidal studies conducted, Peperomia pellucida phytochemical has significance antibacterial activity against clinical Streptococcus pneumoniae, Escherichia coli.
THE PHYTOCHEMICAL SCREENING AND ANTIOXIDANT POTENTIAL OF CINNAMOMUM JAVANICUM BLUME LEAVES from CENTRAL KALIMANTAN / المسح الكيميائي النباتي والفاعلية كمضادات الأكسدة لأوراق سينامومم جافانيكم بلوم من كاليمانتان الوسطى
قطاع الدراسات الصيدلية / العقاقير
تخصص البحث : العقاقير
Bulletin of Pharmaceutical sciences / / vol.44 الجزء الأول - 1/7/2021
تاريخ تقديم البحث 19/08/2021
تاريخ قبول البحث 26/08/2021
عدد صفحات البحث 8
سارة يوسف محمد( Sarahyousef30@yahoo.com - )
د/ سياهردا ديان ارهانى - مؤلف رئيسي
العقاقير
Background: Sintok lancang (Cinnamomum javanicum Blume.) is one of the typical plant of Central Kalimantan, which has not been widely studied. Local people use leaves of C. javanicum to treat various diseases, like diabetes and skin diseases. Based on this, it is necessary to do preliminary study to know phytochemical content and antioxidant potential that may be contained in this plant. Methods: C. javanicum leaves were extracted using percolator with 96% ethanol. The extract was tested for qualitative phytochemical with standard procedures, while the antioxidant test was carried out using 1,1-diphenyl-2-picrylhydrazyl (DPPH) method with quercetin as standard and ferric reducing antioxidant power (FRAP) with trolox as standard. Results: The result showed ethanolic extract of C. javanicum leaves qualitative phytochemical contained alkaloid, flavonoid, tannin and steroid. Antioxidant activity test showed leaves of C. javanicum with DPPH method IC50= 26.99±0.27 ppm and antioxidant activity with FRAP method 779.73±19.66 µmoltrolox/g. Conclusion: It can be concluded that C. javanicum leaves have potential high antioxidant activity was tested with DPPH and FRAP method. Furthermore need further research, especially to determine specific compound of C. javanicum leaves.
EVALUATION OF PHARMACOTHERAPY PROTOCOLS FOR COVID 19 PATIENTS IN BAQIYATALLAH HOSPITAL AND THEIR OUTCOMES / تقييم بروتوكولات العلاج الدوائي لمرضى كوفيد 19 ونتائجها في مستشفى بقية الله بطهران
قطاع الدراسات الصيدلية / الصيدلة الأكلينيكية
تخصص البحث : الصيدلة الأكلينيكية
Bulletin of Pharmaceutical sciences / / vol.44 الجزء الأول - 1/7/2021
تاريخ تقديم البحث 19/08/2021
تاريخ قبول البحث 26/08/2021
عدد صفحات البحث 9
سارة يوسف محمد( Sarahyousef30@yahoo.com - )
د/ فريدة بهرام - مؤلف رئيسي
الصيدلة الإكلينيكية
Purpose: The propagation of the new coronavirus, COVID 19, is still a major priority for many countries around the world. Because of the lack of effective certain antiviral therapy for COVID 19. Special medicinal protocols are needed in medical centers to reduce the mortality rate. Methods: Due to constant change of national pharmacotherapy protocols for COVID-19 patients, this study compares the outcomes of three specific pharmacotherapy regimens (Table 1) and the combination of all 3 regimens for management of 614 COVID-19 patients hospitalized in Baqiyatallah hospital in Tehran, this hospital became a referral centers for receiving COVID 19 patients in Iran. Results: The more reliable result of treatments belongs to regimen 3 which indicated 98.26% recovered patients and 1.15% mortality rate and the lowest period of hospitalization with 4.4±0.21 days’ duration. Conclusion: It seems that regimen 3 (with a special combination of antiviral and Azithromycin ant anti-inflammatory drugs) had the best result in improving patients with COVID 19 and this result has not conflict with the comorbidity status of patients.
THE STATUS OF COMMUNITY PHARMACY IN EGYPT: ARE COMMUNITY PHARMACIES READY TO DELIVER CLINICAL PHARMACY SERVICES AT THE NATIONAL LEVEL? / وضع الصيدلة المجتمعية في مصر: هل الصيدليات المجتمعية جاهزة لتقديم خدمات الصيدلة الأكلينيكية على المستوى الوطني؟
قطاع الدراسات الصيدلية / الصيدلة الأكلينيكية
تخصص البحث : الصيدلة الأكلينيكية
Bulletin of Pharmaceutical sciences / / vol.44 الجزء الأول - 1/7/2021
تاريخ تقديم البحث 19/08/2021
تاريخ قبول البحث 26/08/2021
عدد صفحات البحث 12
سارة يوسف محمد( Sarahyousef30@yahoo.com - )
أ.د/ محمد محمود عبداللطيف - مؤلف رئيسي
الصيدلة الإكلينيكية
Pharmacy practice is witnessing considerable changes in concept and practice in Egypt in recent years. Egypt has the highest number of pharmacists per capita and community pharmacies in the Middle East and North Africa region. Literally, all medicines are freely sold without a prescription irrespective of their status as prescription drugs or over-the-counter drugs; and the pharmacist plays a key role in recommending basic medicines to the public. Community pharmacies are widely distributed throughout the country and more accessible healthcare facility to the community that offering clinical, diagnostic and public health services. The concept of pharmaceutical care has not also been acknowledged yet at community pharmacies; and most community pharmacies lack computerized systems and modern technologies, as well as lack of qualified pharmacists who could deliver clinical pharmacy services. Despite laws and regulations exist; there is a lack of enforcement of those regulations to ensure good pharmacy practice at community pharmacies. The aim of the review was to describe the current status of community pharmacy in Egypt and their preparedness to deliver clinical pharmacy services.
HUMAN PLATELET LYSATE IS A GOOD ALTERNATIVE TO FETAL BOVINE SERUM IN BONE MARROW KARYOTYPING MEDIUM / حُلاّلة الصفيحات البشريَّة كبديل جيد عن المصل الجنيني البقري في أوساط الزرع المستخدمة في التنميط الصبغي لعينات نقي العظام
قطاع الدراسات الصيدلية / الصيدلانيات
تخصص البحث : الصيدلانيات
Bulletin of Pharmaceutical sciences / / vol.44 الجزء الأول - 1/7/2021
تاريخ تقديم البحث 19/08/2021
تاريخ قبول البحث 26/08/2021
عدد صفحات البحث 8
سارة يوسف محمد( Sarahyousef30@yahoo.com - )
د/ عبدالقادر ميما - مؤلف رئيسي
الصيدلانيات
Fetal bovine serum (FBS) is frequently used as a growth factor and as a source of proteins in culture media, but it may contain pathogens.In this study, we aimed to evaluate the possibility of using human platelet lysate (HPL) as a substitute for FBSin bone marrow karyotyping medium. The study included 30 samples of bone marrow aspiration from patients attending the Aleppo University Hospital. Our results showed that the concentration of IGF-1 and GH in HPL was (117 ng/ml) and (2.6 mIU/L) respectively.We also determined the mitotic index (MI), and the results showed that MI values were higher when using the medium supplied with HPLcompared to medium supplied with FBS.Statistical study also showed that there were significant differences (p= 0.001) in MI values when comparing the two media. Our results suggest that HPLcan be used as a substitute for FBSin bone marrow karyotyping medium.
ROSIGLITAZONE SHOWS SEQUENCE-SPECIFIC SYNERGY WITH CHEMOTHERAPEUTIC DRUGS IN INHIBITION OF MCF-7 BREAST CANCER CELL LINE / عقار الروزيجليتازون يظهر تآزرًا خاصًا بالتسلسل مع أدوية العلاج الكيميائي في تثبيط خط خلايا سرطان الثدي MCF-7
قطاع الدراسات الصيدلية / الكيمياء الحيوية
تخصص البحث : الكيمياء الحيوية
Bulletin of Pharmaceutical sciences / / vol.44 الجزء الأول - 1/7/2021
تاريخ تقديم البحث 19/08/2021
تاريخ قبول البحث 26/08/2021
عدد صفحات البحث 12
سارة يوسف محمد( Sarahyousef30@yahoo.com - )
د/ سالى المعلم - مؤلف رئيسي
الكيمياء الحيوية
Preclinical studies have shown that peroxisome proliferator-activated receptor ? (PPAR?) ligands such as thiazolidinediones (TZDs) can exert antitumor effects against breast cancer and a variety of other cancers. In this study, we investigated the potential of repurposing a PPAR? ligand, rosiglitazone (RGZ), in combination with either of three chemotherapeutic agents, doxorubicin (Dox) or cisplatin (Cis) or 5-fluorouracil (5-FU), for the in-vitro treatment of breast cancer cell line, MCF-7. RGZ augmented the growth inhibition effect of Cis and 5-FU on MCF-7 cells. The synergy was observed only when chemotherapy preceded RGZ and not vice versa, demonstrating a sequence-specific effect. We also observed that the first administered drug gave its cell cycle pattern and apoptosis/necrosis pattern to the subsequently applied drug. Besides, no adipose differentiation in the form of lipid droplet accumulation was induced in treated cells regardless of the used drugs and their sequence of application. Together, our data show a synergistic effect of administering RGZ after Cis or 5-FU and suggests an inhibitory role of RGZ on the chemo-resistant MCF-7 side-populations.
ASSESSING THE EFFECTS OF ORLISTAT AS AN ANTI-OBESITY DRUG IN HIGH FAT DIET INDUCED OBESITY IN MALE RATS / تقييم تأثير الأورليستات كعقار مضاد للسمنة على السمنة المستحثة بنظام غذائى عالى الدهون فى ذكور الجرذان
قطاع الدراسات الصيدلية / General
تخصص البحث : General
Bulletin of Pharmaceutical sciences / / vol.44 الجزء الأول - 1/7/2021
تاريخ تقديم البحث 19/08/2021
تاريخ قبول البحث 26/08/2021
عدد صفحات البحث 8
سارة يوسف محمد( Sarahyousef30@yahoo.com - )
د/ إيناس صالح عبدالباقى - مؤلف رئيسي
الكيمياء الحيوية والأدوية والسموم
The present work was designed to evaluate the effectiveness of the orlistat drug on some hormones and biochemical parameters in male rats received a high fat diet (HFD). Twenty-four rats were divided into four groups: 1st group administered normal diet, 2nd group administered HFD, the 3rd group administered HFD plus 9.5 mg/kg b.w./day orlistat, and the 4th group administered HFD diet plus 19 mg/kg b.w./day orlistat. The experimental period was for four weeks. At the end of the experiment, blood samples were obtained for biochemical and hormonal assays. The administration of HFD for four weeks increased significantly the weight gain, serum total cholesterol (TC), triglycerides (TAG), low density lipoprotein (LDL-c), glucose, insulin, triiodothyronine (T3), thyroxine (T4), leptin, with the significant decrease in high density lipoprotein (HDL-c), ghrelin, thyrotropin (TSH) and testosterone hormones in the serum as compared with the control group. The treatment with orlistat neutralized the levels of the measured parameters as compared with HFD fed rats and the results were correlated with the dose of orlistat. In conclusion, an improvement was observed in the biochemical and hormonal results by the treatment with orlistat drug.
CYTOTOXIC EFFECT OF DEFINE CONCENTRATION OF YARROW (ACHILLEA MILLEFOLIUM) EXTRACT USED IN IRANIAN TRADITIONAL MEDICINE ON AGS HUMAN GASTRIC CANCER CELL-LINE / التأثير السام للخلايا من تركيز محدد من مستخلص القيصوم الألفي الأوراق (الحزنبل) المستخدم في الطب التقليدي الإيراني على خط خلايا سرطان المعدة البشري
قطاع الدراسات الصيدلية / General
تخصص البحث : General
Bulletin of Pharmaceutical sciences / / vol.44 الجزء الأول - 1/7/2021
تاريخ تقديم البحث 19/08/2021
تاريخ قبول البحث 26/08/2021
عدد صفحات البحث 10
سارة يوسف محمد( Sarahyousef30@yahoo.com - )
د/ ماجد مرجانى - مؤلف رئيسي
الكيمياء الحيوية والعقاقير
Gastric cancer is one of the main cancer-related death causes in the world threatening almost 12 million human lives by 2020. Achillea millefolium L. (yarrow) with domestic Iranian name of Bumadaran, has been as a curative plant for several medical conditions for a long time. Its antimicrobial and wound healing effects have been reported and in this study, we aimed to survey the in-vitro cytotoxic and anti-cancer effects of this plant. After taxonomically identification of Achillea millefolium L., its hydroalcoholic extract was extracted and the AGS gastric cancer and L-929 normal fibroblastic cell-lines with treated by the different concentrations of extract in 3 time periods (24, 48, and 72 hrs). MTT assay was performed for the evaluation of cytotoxic effects. The 24 hrs treatment did not affect cell survival, notably, while the concentrations of 64 and 16 µg/ml were determined as IC50 concentrations at 48 and 72 hrs incubation times respectively. The 72 hrs incubation time with 16 µg/ml showed the best effectiveness on cancerous cell-line while being safe for normal cell-line. The long-term treatment of AGS cancer cell-line by low concentrations of yarrow extract could be useful for the cytotoxicity upon this type of cancerous cells.
COMPARATIVE STUDY OF THE EFFECTS OF ZOUSH OINTMENT AS A NATURAL PRODUCT AND SLIVER SULFADIAZINE ON THE SECOND-DEGREE BURN WOUNDS HEALING IN MICE: ROLE OF ANTIOXIDANTS AND THE GENE EXPRESSION OF MATRIX METALLOPROTINASE-9 / دراسة مقارنة لتأثيرات مرهم ZOUSH كمنتج طبيعي وسلفاديازين الفضة على التئام جروح الحروق من الدرجة الثانية في الفئران: دور مضادات الأكسدة والتعبير الجيني لمصفوفة ميتالوبروتيناز-9
قطاع الدراسات الصيدلية / الكيمياء الحيوية
تخصص البحث : الكيمياء الحيوية
Bulletin of Pharmaceutical sciences / / vol.44 الجزء الأول - 1/7/2021
تاريخ تقديم البحث 19/08/2021
تاريخ قبول البحث 26/08/2021
عدد صفحات البحث 12
سارة يوسف محمد( Sarahyousef30@yahoo.com - )
د/ زهرى جانجرافى - مؤلف رئيسي
الكيمياء الحيوية
Despite progress in the treatment of burn wounds, effective wound healing remains a severe challenge. In present study, the efficacy of ZOUSH ointment on second-degree burn wound in an animal model has been investigated through antioxidant analysis. The expression of Mmp-9 as a repair factor was measured as well. After induction of second-degree burn, eighty female BALB/C mice were randomly divided into four groups. The first group was treated with ZOUSH, the second group received Silver Sulfadiazine (SSD), the third group received Eucerin, and the control group no medication. Every five days, skin and blood samples of five sacrificed mice were collected and stored at -80°C for further biochemical and histological analyses. Results showed a significant increase in Superoxide Dismutase (SOD), Catalase (CAT), and Glutathione (GSH.); however, Malondialdehyde (MDA) concentration significantly decreased in both samples of the ZOUSH treated groups in comparison with the control group (p? 0.05). Evaluating the wound healing process during the treatment showed that ZOUSH accelerates the wound healing process compared to other groups. Gene expression analysis showed increased Mmp-9 expression level more than ten times compared to the untreated group. According to present study results, ZOUSH ointment proved highly effective in the wound healing of the second-degree burn. Thus, ZOUSH ointment seems to be a reliable alternative to current burn wound therapies.
IDENTIFICATION AND charACTERIZATION OF METALLO-?-LACTAMASES PRODUCING PSEUDOMONAS AERUGINOSA CLINICAL ISOLATES IN AL-AZHER UNIVERSITY HOSPITAL, ASSIUT / تحديد وتوصيف الميتالوبيتالاكتاميز المنتج من عزلات ميكروبات السيدوموناس أيروجينوزا السريرية في المستشفى الأزهر الجامعي
قطاع الدراسات الصيدلية / الميكروبيولوجيا الطبية والمناعة
تخصص البحث : الميكروبيولوجيا الطبية والمناعة
Bulletin of Pharmaceutical sciences / / vol.44 الجزء الأول - 1/7/2021
تاريخ تقديم البحث 19/08/2021
تاريخ قبول البحث 26/08/2021
عدد صفحات البحث 12
سارة يوسف محمد( Sarahyousef30@yahoo.com - )
د/ رحاب صلاح عبدالرحمن موسى - مؤلف رئيسي
الميكروبيولوجيا
Purpose: Study aimed to determine the frequency of nosocomial infections caused by P. aeruginosa and its distribution among different intensive care units (ICUs), to determine the antibiotic resistance pattern, and to determine the prevalence of Metallo-?-lactamase (M?L) among isolates by phenotypic methods. Methods: A total of 74 P. aeruginosa isolates were identified from different clinical specimens in AL-Azher University Hospital. The antimicrobial susceptibility was tested by disk diffusion (Kirby-Bauer) method and phenotypic screening for M?Ls was performed using Combined Disk Test (CDT) and double-disk synergy test (DDST). Results: The frequency of P. aeruginosa isolates from clinical specimens was 18% (74/412). The highest sensitivity was to imipenem 38 isolates (51.3%). The antibiotic sensitivity was in descending manner to meropenem (48.6%) > levofloxacin (28.4%) > ciprofloxacin and ceftazidime (21.6%), while the highest resistance rates were to carbenicillin 70 isolates (94.6%) then gentamicin (70.3%). Out of 74 P. aeruginosa isolates, 51/74 (69%) strains were multidrug resistant (MDR), based on the CDT results 26/74 (35.1%) isolates and by DDST 23/74 (31.1%) isolates were confirmed to be M?Ls producers. Conclusion: There is a growing risk for isolation of MDR P. aeruginosa among the MBL-producer isolates suggests the need for continuous assessment of antimicrobial susceptibility and surveillance of antibiotic prescription. In addition, infection control measures are needed to prevent further dissemination of these organisms
INFLUENCE OF DIFFERENT RESTORATIVE MATERIALS ON THE SENSITIVITY OF STREPTOCOCCUS MUTANS BIOFILM TO DIFFERENT ANTIMICROBIAL MOUTHRINSES IN-VITRO / تأثير المواد الترميمية المختلفة على حساسية بيوفيلم العقديات الطافرة لتأثير الغسولات المضادة للجراثيم فى المختبر
قطاع الدراسات الصيدلية / General
تخصص البحث : General
Bulletin of Pharmaceutical sciences / / vol.44 الجزء الأول - 1/7/2021
تاريخ تقديم البحث 19/08/2021
تاريخ قبول البحث 26/08/2021
عدد صفحات البحث 12
سارة يوسف محمد( Sarahyousef30@yahoo.com - )
د/ نيرمين الآغا - مؤلف رئيسي
الكيمياء الحيوية
Objectives: The aim of this study was to assess the antibacterial effects of chlorhexidine gluconate, sodium fluoride, and sodium fluoride-xylitol combination mouthrinses on S. mutans biofilm in-vitro, and most significantly, we aimed to compare different restorative materials regarding the sensitivity of S. mutans biofilms formed on their surfaces to the antibacterial effects of the tested mouthrinses. Results: Chlorhexidine gluconate 0.12%, sodium fluoride 0.05%, and sodium fluoride 0.05% - xylitol 25% combination exhibited statistically significant antibacterial effects on S. mutans biofilm. The addition of xylitol 25% to sodium fluoride 0.05% mouthrinse was associated with higher antibacterial effects compared with sodium fluoride alone. S. mutans biofilms formed on GIC specimens exhibited the highest sensitivity to antibacterial treatments, whereas biofilms formed on stainless steel specimens showed the lowest sensitivity. Conclusion: S. mutans biofilms formed on different restorative materials exhibited different sensitivity levels to antimicrobial mouthrinses.
PLASMA EPSTEIN-BARR VIRUS (EBV) DNA AS A BIOMARKER FOR DIAGNOSIS OF SYRIAN EBV-POSITIVE BURKITT’S LYMPHOMA / الحمض النووي لفيروس إبشتاين بار (EBV) كمؤشر بيولوجي لتشخيص لمفومة بيركيت إيجابية EBV فى سوريا
قطاع الدراسات الصيدلية / General
تخصص البحث : General
Bulletin of Pharmaceutical sciences / / vol.44 الجزء الأول - 1/7/2021
تاريخ تقديم البحث 19/08/2021
تاريخ قبول البحث 26/08/2021
عدد صفحات البحث 10
سارة يوسف محمد( Sarahyousef30@yahoo.com - )
د/ رنا حبيب - مؤلف رئيسي
الكيمياء الحيوية
Epstein-Barr virus - positive Burkitt’s Lymphoma is defined as the presence of Epstein-Barr virus (EBV) in tumor cells, the standard way to detect (EBV) in Burkitt’s Lymphoma is in-situ hybridization (ISH) of EBV-encoded small RNA (EBERs) in tumor cells. The present study aimed to evaluate plasma Epstein-Barr virus (EBV) DNA as a noninvasive biomarker for diagnosis and prognosis of EBV-positive Burkitt’s lymphoma. The study included 40 newly diagnosed patients with Burkitt’s lymphoma, ranging in age from 4 to 60 years, and 55 sex and age-matched controls. Forty formalin-fixed paraffin-embedded blocks of Burkitt’s lymphoma tissue samples were used to investigate the EBV by in-situ hybridization detection of the EBERs. Plasma EBV DNA was quanti?ed by real-time quantitative polymerase chain reaction (PCR) for all Burkitt lymphoma patients prior to therapy and for control. The results showed that (22/40, 55%) of Burkitt lymphoma were positive for histological EBER, whereas plasma EBV DNA was detectable (range from 1.2×104 to 4.7×106 copies/mL) in all EBV-positive Burkitt lymphoma samples (22/22). EBV DNA was undetectable in all cases of EBV-negative Burkitt lymphomas (18/18) and all healthy control (55/55). It is worth mentioning that our results demonstrated that the EBV DNA load was significantly high in the EBV-positive BL patients suffering poor prognostic state. In conclusion: Plasma EBV-DNA can be used as a noninvasive biomarker for diagnosis and prognosis of EBV-positive Burkitt’s lymphoma.
PRELIMINARY INVESTIGATION ON THE PRODUCTION OF SURFACE ACTIVE COMPOUNDS WITH ANTIMICROBIAL AND EMULSIFYING PROPERTIES BY BACILLUS CLAUSII AND LACTOBACILLUS RHAMNOSUS PROBIOTIC STRAINS / التحقيق الأولي في إنتاج مركبات نشطة السطح مع خصائص مضادة للميكروبات وخصائص استحلابية بواسطة سلالات الكائنات الحية المجهرية Bacillus clausii و Lactobacillus rhamnosus
قطاع الدراسات الصيدلية / الميكروبيولوجيا الطبية والمناعة
تخصص البحث : الميكروبيولوجيا الطبية والمناعة
Bulletin of Pharmaceutical sciences / / vol.44 الجزء الأول - 1/7/2021
تاريخ تقديم البحث 19/08/2021
تاريخ قبول البحث 26/08/2021
عدد صفحات البحث 6
سارة يوسف محمد( Sarahyousef30@yahoo.com - )
د/ ماركوس فينيسيوس دياس سوزا - مؤلف رئيسي
الميكروبيولوجى
Surface active compounds (SACs) of bacterial origin are amphiphilic heteropolymers that may alter the interaction of gas-liquid, solid-liquid, and immiscible liquids surfaces, decrease the surface and interfacial tensions of liquids, and form stable emulsions. SACs are divided in two main groups: low molecular weight, the biosurfactants, and high molecular weight, the bioemulsifiers. Probiotics are live microorganisms that, when administered in determined levels, provide benefits to the host. They can produce SACs with several properties, such as the emulsification and solubilization of substrates and antimicrobial activity. Here we show that the cell-free supernatant of commercial probiotic strains of Bacillus clausii (formerly Bacillus subtilis), a spore-forming species that can tolerate biliary salts and survive at acid gastric conditions, and Lactobacillus rhamnosus, a lactic acid facultative heterofermentative bacterial species, are able to emulsify apolar fluids including gasoline fuel, suggesting the presence of biomolecules that could be explored for bioremediation. L. rhamnosus also presented antimicrobial activity, whereas Bacillus clausii did not.
OPTIMIZATION OF CULTURE CONDITIONS FOR KOJIC ACID PRODUCTION IN SURFACE FERMENTATION BY ASPERGILLUS ORYZAE ISOLATED from WHEAT GRAINS / تحسين ظروف الاستزراع لإنتاج حمض الكوجيك في التخمير السطحي بواسطة أسبيرجلس اوريزى المعزولة من حبوب القمح
قطاع الدراسات الصيدلية / General
تخصص البحث : General
Bulletin of Pharmaceutical sciences / / vol.44 الجزء الأول - 1/7/2021
تاريخ تقديم البحث 19/08/2021
تاريخ قبول البحث 26/08/2021
عدد صفحات البحث 11
سارة يوسف محمد( Sarahyousef30@yahoo.com - )
د/ عبدالحميد محمد رسمى - مؤلف رئيسي
النبات والميكروبيولوجى
Kojic acid is a natural organic acid synthesized during aerobic fermentation of carbohydrates as a secondary metabolite by some species of Aspergillus and has been used commercially in several industrial applications. The current investigation aims to optimize the culture conditions for kojic acid production from starch as a carbon source by the novel isolate Aspergillus oryzae 1034. Seventy-five isolates representing 11 species of Aspergillus were isolated from stored wheat grains and screened for kojic acid biosynthesis. Amongst, A. oryzae 1034 was selected as the most potent kojic acid producer from starch. This strain was subjected to different fermentation conditions to maximize the kojic acid production from starch versus glucose. The results concluded that glucose and starch substrates in concentrations 60 and 80 g/l, respectively were the optima for kojic acid production. The optimum phosphorus concentration was 0.5 and 2.0 g/l KH2PO4 in glucose and starch media, respectively. The maximum kojic acid yield was attained at 28?C for 11 days of incubation in both glucose and starch media with pH 4.5 and 5.0, respectively. Supplementation of Pb+2 to glucose medium and Zn+2 to starch medium stimulated the biosynthesis of kojic acid to 79.3 and 68.8 g/l, respectively. Alternatively, kojic acid biosynthesis was decreased by supplementation of amino acids in the fermentation medium. These findings suggest the possibility of using A. oryzae 1034 as a promising.
VIRULENCE PROFILE OF HELICOBACTER PYLORI DETECTED IN GASTRIC BIOPSIES OF PATIENTS UNDERGOING ENDOSCOPY IN UPPER EGYPT / ملامح أوجه شراسة الهيليكوباكتر بيلوري التي تم اكتشافها في خزعات المعدة للمرضى الذين يخضعون للتنظير في صعيد مصر
قطاع الدراسات الصيدلية / الميكروبيولوجيا الطبية والمناعة
تخصص البحث : الميكروبيولوجيا الطبية والمناعة
Bulletin of Pharmaceutical sciences / / vol.44 الجزء الأول - 1/7/2021
تاريخ تقديم البحث 19/08/2021
تاريخ قبول البحث 26/08/2021
عدد صفحات البحث 12
سارة يوسف محمد( Sarahyousef30@yahoo.com - )
د/ سحر أحمد مندور - مؤلف رئيسي
الميكروبيولوجيا
Background: Helicobacter pylori (H. pylori) is a common cause of gastric ulcers and is a risk factor for gastric carcinoma. Little data are available about the characters of H. pylori causing infections in patients from Upper Egypt. Therefore, the aim of this study was to investigate the frequency of H. pylori infection in Egyptian patients undergoing endoscopy and complaining of persistent upper gastrointestinal symptoms directly in biopsy specimens using PCR technique and to study the associated virulence-related genes in H. pylori positive samples. Materials and Methods: One hundred and twenty gastric biopsy specimens were collected from Egyptian patients admitted to Assiut University Hospital and complaining of persistent upper gastrointestinal symptoms. H. pylori DNA was extracted for molecular identification by PCR. Positive samples were further analyzed to investigate the presence of different virulence-related genes and gene combinations. Results: H. pylori was detected in 92/120 (76.6%) of collected biopsies. All strains carried the vacA s1 subtype. The prevalence of the virulence genes cagA, cagE, iceA1, iceA2 and oipA were 79 (84.8%), 41(44.5%), 69 (75%), 44 (47.8%), and 55(59.7%) of the H. pylori positive samples, respectively. Genetic analysis showed that H. pylori were grouped into 29 different genotype combinations. The s1/m1/i1/d1/c1/cagA/iceA1/oipA genotype was the most predominant. Conclusion: Our results show a high frequency of H. pylori infections among Egyptian patients with gastrointestinal symptoms. The s1/m1/i1 vacA hybrid is the most prevalent subtype. The distribution of the different virulence-related genes shows alarming rates of cagA, cagE, iceA1, iceA2 and oipA. Careful monitory of H. pylori infections and investigation of their genetic characters should be carried out to control the spread of the virulent strains.
CHARACTERIZATION, IN-VITRO EVALUATION OF PROBIOTIC POTENTIAL AND ANTAGONISTIC ACTIVITY OF selectED LACTIC ACID BACTERIA STRAINS ISOLATED from NATURAL ORIGIN AGAINST SOME HUMAN PATHOGENS / فحص إمكانات عزلات مختارة من سلالات حمض اللبنيك المعزولة من اصول طبيعية كبروبيوتيك وتوصيفها وتقييمها مختبريا للنشاط المضاد للميكروبات ضد بعض مسببات الأمراض البشرية
قطاع الدراسات الصيدلية / الميكروبيولوجيا الطبية والمناعة
تخصص البحث : الميكروبيولوجيا الطبية والمناعة
Bulletin of Pharmaceutical sciences / / vol.44 الجزء الأول - 1/7/2021
تاريخ تقديم البحث 19/08/2021
تاريخ قبول البحث 26/08/2021
عدد صفحات البحث 17
سارة يوسف محمد( Sarahyousef30@yahoo.com - )
د/ عزة زكريا - مؤلف رئيسي
الميكروبيولوجيا
Probiotic bacteria have recently become popular for their health-related beneficial effects. In this study, 19 lactic acid bacteria were isolated from breast milk, dairy products, infant stool, vaginal swabs, fermented beverages, and fermented grapes. These isolates were evaluated for their tolerance to gastrointestinal conditions, hydrophobicity, auto-aggregation, and antimicrobial activity. Five isolates, belonging to Pediococcus spp. and Lactobacillus spp., showed potential probiotic features. These isolates were tested for the presence of virulence enzymes and for susceptibility to various antibiotics. The isolates were found to be non-hemolytic and lacked gelatinase and deoxyribonuclease enzymes, however, some antibiotic resistance genes were detected. To ensure the safety of the used probiotics, cell-free supernatants (CFS) of the promising candidates were prepared and its antimicrobial activity before and after neutralization was assessed against standard strains of Candida albicans, Gram-positive, and Gram-negative bacteria. The neutralization of the CFS significantly diminished the antibacterial and antifungal activities of probiotics. Using the time-kill assay, the combination of CFS of Lactiplantibacillus plantarum with gentamicin and ceftazidime was tested against Staphylococcus aureus and Escherichia coli clinical isolates. A significant reduction in log number of survivors was obtained with gentamicin against E. coli and with ceftazidime against both isolates. The anti-biofilm ability of the CFS of the selected probiotics was tested as well. Neutralized CFS caused 26-52% inhibition of S. aureus isolate biofilm formation compared to the control. In conclusion, the five selected isolates could be considered promising probiotic candidates that can be used as biotherapeutics in case of bacterial infection.
ANTIMICROBIAL PEPTIDES AS POTENT COMPOUNDS FOR REDUCTION OF COVID-19 INFECTION / الببتيدات المضادة للميكروبات كمركبات فعالة للحد من عدوى كوفيد-19
قطاع الدراسات الصيدلية / الميكروبيولوجيا الطبية والمناعة
تخصص البحث : الميكروبيولوجيا الطبية والمناعة
Bulletin of Pharmaceutical sciences / / vol.44 الجزء الأول - 1/7/2021
تاريخ تقديم البحث 19/08/2021
تاريخ قبول البحث 26/08/2021
عدد صفحات البحث 10
سارة يوسف محمد( Sarahyousef30@yahoo.com - )
د/ محلاجة زاهدى - مؤلف رئيسي
الميكروبيولوجى
COVID-19 is an infectious disease caused by a new identified coronavirus in china, SARS-COV2. There are no efficient treatments for COVID-19. Therefore, it is essential to investigate new therapies for this problem. Due to specific mechanism for inhibition of microbial growth, antimicrobial peptides can be considered as one of the best therapies in this field. Antimicrobial peptides (AMPs) are important agents that are made by the immune system in response to pathogens. This kind of immune response exists in all animal categories from prokaryotes to humans. Different types of AMPs have been identified and isolated from various organisms from bacteria to humans. So far, 190 antiviral peptides with antiviral effects have been extracted and introduced from various animal sources. These natural compounds and their derivatives, e.g. synthetic peptides, can be considered as new therapeutic goals in COVID-19. In this review, we assessed these peptides in different animal categories as well as synthetic peptides and the possibility of using these compounds in the treatment of COVID-19.
EVALUATION OF ANTINOCICEPTIVE EFFECT OF THE KETOROLAC-TOPIRAMATE COMBINATION IN THE RAT FORMALIN TEST / تقييم التأثير المضاد للالتهاب لمزيج كيتورولاك - توببيراميت في اختبار الفورمالين بالجرذان
قطاع الدراسات الصيدلية / General
تخصص البحث : General
Bulletin of Pharmaceutical sciences / / vol.44 الجزء الأول - 1/7/2021
تاريخ تقديم البحث 19/08/2021
تاريخ قبول البحث 26/08/2021
عدد صفحات البحث 11
سارة يوسف محمد( Sarahyousef30@yahoo.com - )
د/ لويس فرناندو - مؤلف رئيسي
علم الأدوية والسموم
Combination therapy approaches to manage acute and chronic pain are commonly used. To characterize the interaction between ketorolac and topiramate in the formalin test, female Wistar rats (200-300 g) were submitted to 1% formalin test. Antinociceptive effect was determined by the administration of ketorolac (3, 10, 30 and 100 mg/kg), topiramate (25, 50, 100 and 200 mg/kg) and their combination by oral route; or ketorolac (25, 50, 100 and 200 µg/paw), topiramate (10, 30, 100 and 300 µg/paw) and their combination by local peripheral route. Isobolographic analysis was used in a fixed dose combination (0.5:0.5) to analyze the nature of the interaction of the combination based on the ED50 of ketorolac (62.8±21.9 mg/kg) and topiramate (24.3±8.5 mg/kg) by oral administration; or the ED30 of ketorolac (67.0±7.97 µg/paw) and topiramate (391.3±51.7 µg/paw) by local peripheral route. Combination of these two drugs significantly reduced the number of flinches in second phase of the test. Theoretical ED50 of the oral combination (ED50T) was 43.5±11.7 mg/kg. Experimentally, the ED50 of the combination (ED50E) had a significantly lower value: 16.7±4.0 mg/kg; indicating the presence of supra additive effects (interaction index was 0.38). For local peripheral route, ED30T was 229.21±64.99 µg/kg. Experimentally, the ED30E had a significantly lower value: 62.00±15.72 µg/paw; indicating synergistic effects (interaction index was 0.27). Results show that oral and local peripheral administration of the combination can interact synergistically to reduce inflammatory pain in the rat formalin test.
INSIGHTS INTO THE IMPACT OF FXR ACTIVATION ON HEPATIC AUTOPHAGY IN A NON-ALCOHOLIC STEATOHEPATITIS MODEL / دراسة تأثير تحفيز مستقبل FXR على الالتهام الذاتي بالكبد في نموذج تجريبي لمرض التهاب الكبد الدهني
قطاع الدراسات الصيدلية / General
تخصص البحث : General
Bulletin of Pharmaceutical sciences / / vol.44 الجزء الأول - 1/7/2021
تاريخ تقديم البحث 19/08/2021
تاريخ قبول البحث 26/08/2021
عدد صفحات البحث 10
سارة يوسف محمد( Sarahyousef30@yahoo.com - )
د/ دلال مصطفى عبدالله - مؤلف رئيسي
الأدوية والسموم
Background: Multiple lines of evidence pointed to the role of dysbiosis, small intestinal bacterial overgrowth and altered intestinal permeability in promoting pro-inflammatory events in the liver leading to the progression of steatosis to non-alcoholic steatohepatitis (NASH). The pivotal involvement of farnesoid-X-receptor (FXR) in maintaining intestinal homeostasis and combating hepatic inflammation was previously established. Nonetheless, the role of hepatic autophagy in NASH pathogenesis and treatment remains controversial. The present study aimed at investigating whether the effect of the FXR agonist, obeticholic acid, on ameliorating NASH-related incidents is related to an impact on hepatic autophagy. Methods: Swiss albino mice were fed an atherogenic high fat diet (Ath-HFD) with dextran sulfate sodium (DSS) in drinking water to induce NASH. Obeticholic acid (5 mg/kg/day, p.o.) was given for 28 days, starting at day 64 post NASH initiation. Histopathological examination of liver and colon samples was performed. Inflammatory markers, IL-1?, IL-6, IFN-? and TNF-?, besides adiponectin, were assessed in the liver. Autophagy genes, ULK1, BECN-1 and ATG5, were assessed by RT-PCR in hepatic tissues. Results: Histopathological changes observed in the liver and colon of the positive control group (Ath-HFD/DSS) were significantly ameliorated after treatment. No noticeable changes were reported in adiponectin and inflammatory markers following treatment except for a partial enhancement in IFN-?. Though ULK1 and BECN-1 gene expression tended to increase after treatment with obeticholic acid compared to Ath-HFD/DSS group, ATG5 mRNA was almost restored. Conclusion: Obeticholic acid ameliorated NASH partially through autophagy and IFN-? enhancements in the liver.
EFFECTS OF ADMINISTRATION OF SIMVASTATIN ON THE THRESHOLD OF THERMAL PAIN TOLERANCE IN ADULT MALE RATS WITH HFD / تأثير تناول السيمفاستاتين على عتبة تحمل الالم الحراري فى ذكور الجرذان البالغين ذوي نظام عذائى عالى الدهون
قطاع الدراسات الصيدلية / General
تخصص البحث : General
Bulletin of Pharmaceutical sciences / / vol.44 الجزء الأول - 1/7/2021
تاريخ تقديم البحث 19/08/2021
تاريخ قبول البحث 26/08/2021
عدد صفحات البحث 7
سارة يوسف محمد( Sarahyousef30@yahoo.com - )
د/ حميد سيبيهرى - مؤلف رئيسي
الفسيولوجى
Background: Increased consumption of high-fat diets has become a global health problem. Obesity by result of various endocrine changes and release of inflammatory cytokines that may contribute to the pathogenesis of pain. Simvastatin is a statin that inhibits the 3-hydroxy-methyl-glutaryl coenzyme A (HMG-CoA) reductase and has anti-inflammatory effects. The aim of the present study was to assess the antinociceptive activity of simvastatin following the use of high-fat diet in Male rats. Methods and materials: Forty adult male Wistar rats divided into six groups: control, high-fat diet, high-fat diet treated with 2 and 20 mg/Kg simvastatin and normal diet treated with 2 and 20 mg/kg simvastatin. Rats were maintained on a high-fat or normal diet for 45 days. Simvastatin was daily given through gavage from two weeks after starting of the study and continued for 30 days. Hot plate and blood biochemistry tests were performed to evaluate the threshold of thermal pain t tolerance. Results: Total cholesterol, LDL, and triglyceride, and body weight in the group that maintained a high fat diet was significantly higher compared with the other groups, especially the control group. But serum TNF-? and IL-6 were not found in a detectable production in all groups. High fat diet significantly reduced thermal pain reaction time. Simvastatin administration significantly decreased thermal hyperalgesia. Conclusion: The results indicate that simvastatin administration is a suitable way for reducing heat hyperalgesia following use of a high fat diet, but there is need to do further research.
جميع البيانات التي يتم تسجيلها على النظام هي ملك للجهة صاحبة هذه البيانات
لا يتم الاطلاع على هذه البيانات من قبل مهندسي المركز إلا للضرورة القصوى ولغرض الدعم الفني وبالاتفاق مع الجهة صاحبة البيانات شفوياً أو كتابياً
لا يتم إعطاء أي بيان من بيانات النظام لأي فرد او جهة فرعية تنتمي للجهة صاحبة البيانات او لأي جهة أخرى إلا بموافقة كتابية من الجهة صاحبة البيانات
يمكن استخدام البيانات المسجلة على النظام بهدف عمل إحصائيات يستفاد منها فى قياس اداء النظام وكفاءة استخدامه من قبل مستخدميه
يتم تأمين جميع البيانات الخاصة بالنظام من خلال تأمين الحاسب الخادم الذي يستضيف البيانات ومن خلال تأمين انتقال البيانات بين جهاز المستخدم والحاسب الخادم وكذلك تأمين واجهة النظام التي يستخدمها المستخدم
كل مستخدم للنظام مسئول مسئولية كاملة عن كلمة المرور الخاصة به وعليه اتخاذ كافة الاحتياطات الممكنة حتى لا يستطيع أى شخص آخر الحصول عليها
تسجيل دخول 
Download Paper
