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العنوان
Effect of Different Levels of Wheatgrass (Triticum
aestivum) on the Immune System Response in the
Experimental Rats /
المؤلف
Elewa, Rahma Saber Kamal.
هيئة الاعداد
باحث / رحمة صابر كمال عليوه
مشرف / إقبال محمود محمد صالح
مشرف / السيدة غندور السيد السحار
مناقش / السيدة غندور السيد السحار
تاريخ النشر
2021.
عدد الصفحات
229 P. :
اللغة
الإنجليزية
الدرجة
ماجستير
التخصص
اقتصاد منزلي
تاريخ الإجازة
1/1/2021
مكان الإجازة
جامعة عين شمس - كلية التربية النوعية - قسم الاقتصاد المنزلي
الفهرس
Only 14 pages are availabe for public view

from 229

from 229

Abstract

Studying the immunity without reference to nutrition is waste of time, not only because enough intakes of nutrients would support the immunity, but also due to the fact that the increase of one nutrient may hinder the effect of another which resulting in immunity depression. Interactions were found between wheatgrass contents antioxidants and protein and other nutrients to enhance immunity
The present work was aimed to investigate the Effect of different levels of wheatgrass (Triticum aestivum) on the immune system response in the experimental rats. The efficiency of the immune system or reactivity in experimental rats beside, the biological effect was evaluated.
In the present study 40male albino rats weighting form 120+-5g (At the beginning of the experiment were performed).the rats were maintained in air-conditioned room on a 12 hours light /dark cycle at 22+2 OC, and acclimatized under the test conditions for one week before treatments.
Rats were fed on basal diet the chemical composition of the given diet was: casein 15%, corn oil 10%, cellulose 5%, vitamin mixture 1%, and salt mixture 4% and corn starch 65%.
Experimental diet and water were offered and libitum all over the Experimental period (6weeks). 40 rats were divided into 8groups, 5 rats in group each, which included two main experiments. In experiment I were fed on basal diet only (positive control group) (Co+) or basal diet + the three prepared food additives groups (1-3)100, 150,200 mg /kg rat weight and at the end of the experimental period (6weeks) the rats were subjected for stress treatment (immune suppression). The analysis was carried out to evaluate the effect of wheatgrass on immune status (as curative effect).
In Experimental II groups (Co-) and groups (4-6)the same trend was used as in the first experiment but the immune suppression (stress) was proceeded just in the beginning of the study (after the adaptation period and before wheatgrass feeding). The analysis of the parameters was preceded each two weeks.
During the feeding experiments, animals were daily inspected and food intake was recorded and body weights were recorded once weeks. Some biochemical evaluations were preceded for animals during the experimental period.
Blood samples were collected form retro orbital plexus form all rats’. Rats narcotized and scarified.
(Hematological analysis, serum protein fractions, liver and kidney function and immunoglobulin). Also effect on lipid peroxidation and Histopathological of spleen Thymus gland, and liver tissues were also studied.
Biological results can be summarized as follows:
1- All groups recorded no significant in initial body weight, finial body weight, body weight gain and food Efficiency (during experimental period).
2- (Preventive groups) recorded in decrease food intake when compared with control group the highest decrease was found in group1, 2 and 3.
3- When (Therapeutic groups) recorded increase in food intake compared to negative control group. The highest increase was found in group6 and5 respectively.
4- All groups recorded no significant in liver weight and spleen weight (during experimental period).
5- All groups receded no significant difference between groups in RBC,S, HB and hematocrit.
6- All groups recorded no significant difference between groups in MCHC.
7- All groups are in difference between groups in RDW and MCH and MCV.
8- MCH were decreased in blood samples of group (1), (3) but increase group (2) in Preventive group when compared to positive control group.
9- MCH was increase in4, 5 and 6 MCH compared to positive control group.
10- All groups recorded decrease MCV were decreased in blood samples of group (1), (3) (2), (4), (5) and 6 compared to control group.
11- All group recorded no significant PLT and MPV in blood samples of group (1), (3) (2), (4), (5) and 6 compared to control group.
12- group 2, 3, 4 and 6 recorded increase in WBC, s compared with control group.
13- Group1and 5 recorded decrease in WBC, s compared with control group.
14- group 1, 2, 3 recorded increases in lymphocyte percentage compared with control group.
15- group 4, 5, 6 recorded decreases in lymphocyte percentage when compared with control group.
16- All groups recorded no significant in NEUT compared with control group.
17- group 2, 3, 5 and 6 recorded increase in monocyte % compared with control group.
• group 1 and 5 recorded decrease in monocyte % when compared to control group.
• All group recorded significant decrease in CHL compared with control group.
• Group2, 3, 4,5and 6 recorded decrease in TG compared with control group.
• group 1 recorded increase in TG compared with control group.
• All group recorded in decrease LDL compared with control group.
• group 1, 4, 5 recorded increases in HDL compared with control group.
• group 6, 2 and 3recorded decrease in HDL compared with control group.
• Group1, 2 and 3 recorded decrease in uric acid and urea when compared with control group.
• Group4, 5 and 6 recorded increase in uric acid and urea when compared with control group.
• Group4, 5, 2 and 6 recorded increase in creatinine when compared with control group.
• Group1, 3 no change in level in creatinine when compared with control group.
• All group recorded no significant in GOT.
• All group recorded significant increase in GPT when compared with control group.
• All group recorded no significant in IGG.
• All group recorded decrease in TP and SOD when compared with control group.
• Studying the Pathology alteration in all groups were d Pathological changes in each of the liver, spleen and thymus glands came in the course of chemical changes. The effectiveness of wheatgrass in strengthening immunity, reducing bad cholesterol, raising the level of good cholesterol and maintaining the level of hemoglobin in the blood
Form the Results of the present study we recommend by:
The high important of wheatgrass have important role hence they play good role in Improve in WBC, s and Immunity booster
Pathological changes in the liver, spleen, and thymus gland came in the course of chemical changes. Encouraging eating wheatgrass to improve immunity, as is the case in reducing harmful cholesterol, raising the level of cholesterol and raising the level of hemoglobin in the blood
We recommend using wheatgrass to help enhancing immunity, as it contains antioxidants and chlorophyll, and it is also recommended for patients with high cholesterol, as it raises good cholesterol, reduces bad cholesterol, and benefits weight loss.