الفهرس | Only 14 pages are availabe for public view |
Abstract Obesity is a common multifactorial public health problem. The worldwide prevalence of obesity nearly tripled between 1975 and 2016. The estimated prevalence of overweight and obesity in Egypt is 61–70% of the adult population. It is also considered as one of the top contributors to mortality among Egyptians. GAD2 gene is one of the studied genes that is suggested to be associated with obesity. It encodes for the 65-kDa isoform of the enzyme GAD2 which catalyzes the formation of GABA from glutamic acid. The GABA in the hypothalamus functions together with NPY in the paraventricular nucleus to stimulate food intake. In addition, GABA serves as a functional regulator of the pancreatic hormonal release. Some researchers hypothesize that single nucleotide polymorphisms (SNPs) in GAD2 gene may enhance the over expression of the GAD2 resulting in overeating and obesity. One of the suggested SNPs in GAD2 gene is the -243A>G polymorphism (rs2236418) where the adenine (A) nucleotide is substituted by the guanine (G) nucleotide at the 243rd position in the non-coding 5’prime promoter region of the GAD2 gene. In the light of the previous data, the aim of the present study was to study the relation between -243A>G polymorphism of GAD2 gene and the presence of obesity.The study was conducted on (30) obese patients with BMI ≥30 kg/m2. In addition, (20) age and sex-matched apparently healthy adults (BMI <25 kg/m2) were included as healthy controls. Detection of -243A>G polymorphism of GAD2 gene was performed by PCR-RFLP technique. The present study revealed that the (AA) genotype and the (A) allele frequencies were significantly higher in healthy controls when compared to obese patients. Meanwhile, the frequencies of the (AG) and (GG) genotypes as well as the (G) allele were significantly higher among obese patients as compared to healthy controls. Data of the present study also revealed no difference between class II and class III obesity as regards the -243A>G SNP genotype distribution or allele frequencies. Finally, no association was observed between the different genotypes of -243A>G SNP and the different demographic, clinical and metabolic features in the studied population. In conclusion, the present study demonstrated the presence of a significant association between the -243A>G polymorphism and obesity. However, the genotype and allele frequencies of this genetic variant did not differ between class II and class III obesity and were not related to any demographic, clinical or metabolic features of the studied population. |