Search In this Thesis
   Search In this Thesis  
العنوان
EFFECT OF PROBIOTIC SUPPLEMENTATION TO FATTENING ANIMAL RATION ON ITS PRODUCTIVE PERFORMANCE /
المؤلف
ELNAGAR, SALAMA ELSAYED ATIA ABO ELENEIN.
هيئة الاعداد
باحث / SALAMA ELSAYED ATIA ABO ELENEIN ELNAGAR
مشرف / Nasr El -Sayed Yahia Mohamed El-Bordeny
مشرف / Ahmed Ragheb Shemeis
مناقش / Gouda Fathi Gouda
تاريخ النشر
2019.
عدد الصفحات
88p. :
اللغة
الإنجليزية
الدرجة
ماجستير
التخصص
العلوم الزراعية والبيولوجية
تاريخ الإجازة
1/1/2019
مكان الإجازة
جامعة عين شمس - كلية الزراعة - انتاج حيوانى
الفهرس
Only 14 pages are availabe for public view

from 88

from 88

Abstract

Probiotic bacteria Microbial strains and growth condition The probiotic bacteria used in this study is a mixture of 15 isolate of lactobacillus sp (Eleven probiotic bacteria isolates were isolated from calves saliva and feces plus 4 isolates were isolated from rumen). The isolates were characterized and identified then propagated to use it as a probiotic supplementation. This work was a part of ”production of probiotic bacteria and evaluation its impact on dairy and beef cattle” funded by STDF (ID: 10802). Lactobacilli isolates were grown on MRS broth (Oxoid) and Streptococci isolates were grown on M17 broth (Difco), after that the broth media incubated for 24 h at 37 °C. The strains were activated two or three times in order to obtain high biomasses in the stationary phase. The present study was divided into two experiments, the first was in vitro experiment and it was aimed to evaluate effect of different level of probiotic supplementation to ruminant rations, using in-vitro batch culture technique to determine degradation and fermentation parameters. The second study was Fattening study this study was to evaluate effect of supplementing ruminant ration with probiotic bacteria supplementation on its productive performance. The best level of probiotic supplementation in the in vitro experiment was subjected for further in vivo investigation. The fattening study divided into to trials, 1st fattening trial and 2nd fattening trial; 1- In vitro experiment In vitro experimental ration was formulated, the ration consisted of 40% alfalfa hay and 60% concentrate feed mixture. Three level of probiotic supplementation (106, 108, 1010 cfu/kg DM) were evaluated. DM and total gas production as well as fermentation parameters of the incubated samples were determined after 24 hrs. of fermentation.
51
SUMMARY AND CONCLUSION
Salama E. El-Nagar, (2019), M.Sc., Fac. Agric., Ain Shams Univ.
Slightly increases (P>0.05) in in-vitro dry matter degradability were observed for the ration supplemented with probiotics bacteria at different levels (106,108 and 1010 cfu/ kg DM) compared to control ration. Probiotics bacteria supplementation with different level (106,108 and 1010 cfu/ kg DM) led to significant (P<0.001) increases in organic matter degradability and total gas production per sample and per g DM, OM, NDF and ADF compared to the not supplemented ration (control ration), and no significant differences were observed among the different levels of probiotics supplementation. Significant increase in total volatile fatty acid concentration after 24 hours’ incubation period compared to the not supplemented ration. On the other hand, the treatment supplemented with probiotic recorded lower ammonia concentration compared to the control group. It could be concluded that, adding probiotics bacteria supplementation to experimental ration resulted increase DM and OM degradability and using dose 106 CFU/kg DM feed is sufficient to induce improvement in degradability and fermentation parameters. 2- Fattining experiment 2.1. 1st fattining trial In the 1st trial, forty-eight male (Friesian) calves with mean initial body weight of about 170.5 ±3.16 Kg were divided into two groups of 24 animals each, each group was assigned randomly to one of two dietary treatments, control or probiotic. The animals were fed total mixed ration (TMR) at rate about 2.3 % of their live body weight in group feeding without or with probiotic 106 CFU per kg DM for control and probiotic groups, respectively. The animals fed rations supplemented with probiotics recorded significantly higher nutrients digestibility as dry matter (DM), organic matter
52
SUMMARY AND CONCLUSION
Salama E. El-Nagar, (2019), M.Sc., Fac. Agric., Ain Shams Univ.
(OM), crude fiber (CF), nitrogen free extract (NFE) and Neutral detergent fiber (NDF) compared to the control group. However, insignificant differences were recorded between the different supplemental groups in Ether extract (EE), Crude protein (CP), Acid detergent fiber (ADF) and Non fiber carbohydrate (NFC). Probiotic supplementation significantly (p≤0.0001) improved, neutral detergent fiber (NDF digestibility. Slightly increase in total digestible nutrients (TDN) and Digestible crude protein (DCP) intake for the group fed ration supplemented with probiotics compared to the control group. Blood plasma total proteins, globulin, triglyceride and creatinine concentration and ALT, AST, alkaline phosphates activity were not significantly (P>0.05) affected by probiotic supplementation to calves ration. No significant differences in final body weight, total gain and average body weight gain, feed conversion as DM, CP and TDN between probiotic group and control group. 2.2. 2nd fattining trial In the 2nd trial, Eighteen (Friesian) calves with mean initial body weight of about 284.93 ±12 Kg were divided into two groups of 9 animals each; each group was assigned randomly to one of two dietary treatments, un-supplemented (control) or supplemented (probiotic). The animals were fed total mixed ration2 (TMR2) consist of 66.6 concentrate feed mixture (CFM) and 33.4 peanut straw without or with probiotic 106 CFU per kg DM for control and probiotic groups, respectively. Slightly increase in DM, Total digestible nutrients (TDN) and Digestible crude protein (DCP) intake for the group fed ration not supplemented with probiotics.
53
SUMMARY AND CONCLUSION
Salama E. El-Nagar, (2019), M.Sc., Fac. Agric., Ain Shams Univ.
Animals fed rations supplemented with probiotics recorded
significantly higher dry matter digestibility compared to the control group.
However, insignificant differences were recorded between the two groups in
OM, CF, NFE, NDF, EE, CP, ADF and NFC.
No significant differences were recorded between the control and
probiotic group in final body weight, total gain and average body weight gain
as well as feed conversion as DM, CP and TDN.
Adding of probiotic bacteria appeared to enhance the Height
measurements by 1.2% for height at hock joint to 4.91 for height at hips.
Notable positive effect for probiotic was recorded for lateral, dorsal body
length and trunk length index, measures related to dairy type animals, by
16.72, 10.13% and 22.4%, respectively. Higher positive significant effects of
probiotic bacteria were observed in present study on width at chest by 36.4%
and width of shoulder by 32.2%.