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العنوان
Potential Cytoprotective Activity of some Natural
and Synthetic Compound(s) against ChemicallyInduced Injury in Human Hepatocytes /
المؤلف
Fahmy,Salma Nasser Mostafa.
هيئة الاعداد
باحث / Salma Nasser Mostafa Fahmy
مشرف / Ashraf B. Abdel-Naim
مشرف / Moushira Ibrahim El-Sayed
مشرف / Ahmed Esmat Abdel Razik
تاريخ النشر
2016
عدد الصفحات
157p.;
اللغة
الإنجليزية
الدرجة
ماجستير
التخصص
العلوم الصيدلية
تاريخ الإجازة
1/1/2016
مكان الإجازة
جامعة عين شمس - كلية الصيدلة - العلوم الصيدلية
الفهرس
Only 14 pages are availabe for public view

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from 157

Abstract

Natural compounds are now considered as a corner stone in
prevention and treatment of various diseases caused by liver injury and
oxidative stress. Resveratrol, its analog Pterostilbene and the synthetic
compound Didox are known as simple antioxidants also showing antiinflammatory effect to protect against liver injury. Pretreatment of HepG2
cells with the tested drugs (Resveratrol, Pterostilbene and Didox) in
concentrations of (10, 100 and 1000 µg/ml) and the standard drug Silymarin
in the concentration of 100 µg/ml prior to CCl4 challenge, protected against
CCl4-induced liver injury in a concentration-dependent manner except for
Didox (10 µg/ml) and Pterostilbene (1000 µg/ml) who showed no significant
difference than CCl4-challenged group. ALT and AST levels were assessed
as an indication of hepatocyte membrane integrity. Furthermore, reduced
glutathione content, SOD, CAT activities and MDA level were measured to
asses oxidative stress level and lipid peroxidation in liver cells. TNF-α, IL-1β
and IL-6 were also measured as an indication of inflammation. As evidenced,
tested drugs in addition to the standard drug Silymarin, showed a significant
decrease in the activity of the medium enzymes ALT and AST. They also
showed significant anti-lipid peroxidation, antioxidant and anti-inflammatory
effects in vitro as evidenced in the significant decrease of MDA, TNF-α, IL-
1β and IL-6 and the significant increase in the enzymatic antioxidants;
CAT,SOD and reduced glutathione content. In conclusion, this study proved
that Resveratrol’s analog “Pterostilbene” has showed the best pattern of
antioxidant and anti-inflammatory effects on CCl4 – challenged HepG2 cells.
This could be explained partly by maintaining hepatic cell integrity,
alleviating oxidative stress resulting in a hepatoprotective effect of the drug
against acute toxicity induced by CCl4.