الفهرس 
Hepatitis C VirusOnly 14 pages are availabe for public view
 |  | from | 144 |  |  |
| | | from | 144 | | |
Abstract
liver diseases are considered to be one o major public health problems worldwide because of the severity of he disease and high morbidity rate. HCV has been identified as the major causative agent of liver diseases in human. the two main cell types involved in cellmediated immunity are T lymphoctes and macrophages. Since IFNgc sis a product of the former and is probably the most important activator of the latter cell type. The aim of the present study was evaluation of interferon gamma in sera of patients with liver diseases immunochemically using specific monoclonal antibody. In this study serum samples of 73 individuals with different pathological disorders of liver diseases were included in the present work; In addition to 12 healthy individuals. The patients were classified into three subgroups:18 noncirrhotic patients. 20 cirrhotic patients. 35 patients with hepatocellular carcinoma (HCC). Detection of HCV antigen in serum of patients using ELISA. 61 out of 73 serum samples with different pathological disorders (84%) were positive for HCV antigen whereas a total of 12 serum samples (16%) were negative. 14 out of 18 noncirrhotic patients (78%), 17 out of 20 cirrhotic patients (85%), 30 out o 35 HCC patients (86%). To determine the antigen (IFNgc? sin the collected serum samples. Serum samples were electrophoresed using SDSPAGE technique and stained with Commassie blue stain. Then proteins from the gel were transferred to nitrocellulose paper using Western blot technique, and then the nitrocellulose paper was exposed to monoclonal antibody against c Myc antigen where one immunoreactive band with 26 kDa appeared in the infected cases only. Indirect ELISA was used to detect IFNgc santigen in the serum samples. IFNgc santigen was detected in 50 out of 73 patients with liver disorders (68.5%). 8 of 18 (44%) noncirrhotic, 16 out of 20 (80%) cirrhosis and 26 out of 35 HCC (74%) patients. Noncirrhotic patients had significantly increased (P<0.05) serum level of IFNgc scompared with those noncirrhotic patients negative for IFNgc? sCirrhotic (P<0.05) and HCC (P<0.001). The relation between serum levels of IFNgc sAND either positive or negative for HCV antigen show that noncirrhotic (P<0.05), cirrhotic (P<0.001) and HCC (P<0.05). from aforementioned results, IFNgc sincreased from noncirrhotic to cirrhotic patients but decreased in HCC. This indicates that following up HCV patients through IFNgc sestimation can be predict the conversion of cirrhotic to HCC. Also increased serum level of IFNgc scan be a characteristic feature of HCV. Furthermore, ELISA remain a promising alternative for sensitive and specific detection of IFNgc?s.