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العنوان
Molecular characterization of multi-drug resistant Campylobacter species isolated from poultry /
المؤلف
Khattab, Rania Hosny Abd EL Aziz.
هيئة الاعداد
باحث / رانيا حسني عبد العزيز
مشرف / محمود عزت السيد
مناقش / سارة يوسف عبد الجليل
مناقش / محمد السيد عناني
الموضوع
poultry. drug.
تاريخ النشر
2023.
عدد الصفحات
137 p. :
اللغة
الإنجليزية
الدرجة
الدكتوراه
التخصص
البيطري
تاريخ الإجازة
19/3/2023
مكان الإجازة
جامعة قناة السويس - كلية الطب البيطري - البكتريا والمناعة والفطريات
الفهرس
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Abstract

In this study, Campylobacter were isolated (182) from (440) broilers and
(440) ballade breeds from Dakahlia governorate that were examined (16%)
C.jejuni and (4%) C.coli.The rate of recovery of campylobacter from
different internal organs showed that high recovery rate was from cloacal
swab, heart, liver, then gizzard as the follow 60%, 24%, 10% then 5%
respectively C.jejuni and 45%, 30%, 14% then 4% C.coli. Morphologically
campylobacter isolates were G-ve, slender spirally curved, the majority
of species are motile,they grow at 37-42Co
,they need 5% O2, 10% CO2,
and 85% N2, it appear dew DROP like colony on mCCDA media.The isolated
campylobacter from chickens were140 C.jejuni and 42 C.coli .API20E
system revealed that99% of suspected isoltes were biochemically identical
to typical campylobacter features .It was clear that all campylobacter
isolates were resistant to Erythromycin, Amoxicillin and Trimmethoprim /
sulphamethoxazole.Campylobacter were sensitive to Meropenem and
Imipenem. PCR assay was carried out for (C.coli and C.Jejuni) to detect
23SrRNA as common gene for C.spp., hipogene for C.Jejuni, Aspgene for
C.coli, betalactamases (Blaoxa), virulencegenes (cadf,
flaA), toxinproductiongene (cdtA,Waac, dnaj), MDRgenes (tetA,tetB),
using specific primer for each gene.Sequencing of gyrA genefor C.Coli and
C.Jejuni .