الفهرس 
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Abstract
GM is a frequently used antibiotic for treating several Gram-negative bacteria. However, 24% of patients who received GM for longer than seven days developed kidney injury.
Oxidative stress is crucial in the development of toxicity induced by GM. As a result, administering antioxidants may assist with minimizing the drug’s possibly dangerous side effects.
PRP is an autologous blood concentrate with a higher platelet concentration than whole blood. PRP contains high amount of platelets, growth factors, clotting factors, cytokines, and chemokines. PRP has protective effect on renal tissue by activating intracellular antioxidant components.
The aim of the present work was to study the possible protective effect of PRP on renal structure in GM- induced nephrotoxicity in adult male albino rats.
This study was carried out on thirty-seven adult albino rat. The rats were divided into:
• Five healthy male rats (weighing about 300 ± 50 g), to obtain the PRP.
• Thirty-two adult male rats (weighing about 200 ± 20 g), were considered as the experimental group. The rats of the experimental group were further divided into 4 groups (8 rats in each group) as following:
group I: Negative control group: without any treatment.
group II: Positive control group, received 1 ml PRP intraperitoneal once.
group III: as GM group received GM (80 mg/kg/day, intraperitoneal, 8 consecutive days).(42)
group IV: as GM+ PRP group received GM for 8 days, and 24 hours later, 1 mL PRP was injected intraperitoneal once.
At the end of induction by GM and 3 days after PRP therapy, blood samples were collected to determine levels of BUN and Cr in serum and the kidneys were removed for tissue processing.
Biochemical findings revealed a significant increase in urea and Cr of GM treated group in comparison to the control group, and there was no significant change between GM+PRP group and the control group.
Histologically, the GM group showed variable degrees of degeneration affecting proximal tubules mainly such as defective brush border, intraluminal hyaline casts, vacuolations, interstitial mononuclear cellular infiltration and vascular congestion. Renal corpuscles were distorted with shrunken or absent glomeruli, while GM+PRP group revealed marked amelioration in the histological structure of the renal tubules and glomeruli.
With Masson’ trichrome stain, there was significant elevation in percentage of fibrosis of renal tissue in GM group in comparison to the control group, and there was significant decrease in percentage of fibrosis of renal tissue in GM+PRP group in comparison with GM group.