الفهرس 
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Abstract
Aim: The following study was performed to determine whether the pumpkin seed oil
(Cucurbita pepo L.) loaded Chitosan nanoparticle (NPSO) has a cytotoxic and apoptotic
effect on tongue squamous cell carcinoma cell line or not.
Methodology: After obtaining the SCC-25 (tongue squamous carcinoma) cells, cell culture
protocol was done according to standard procedures. The cultures were incubated at 37°C
for 24 hrs, then their treatment with the serial concentrations of the Nano chitosan loaded
pumpkin seed oil and Taxol drug, after that incubation is carried out for 48 h at 37ºC.
Chitosan/pumpkin seed oil nanoparticles were prepared using ionotropic gelation at room
temperature. Caspase 9 enzyme essay was carried out using ROBONIK P2000 ELISA
READER wl 450 nm.
Results: The results of the MTT cell cytotoxicity assay showed that there is an inverse
relation between the serial NPSO_CH concentrations and Taxol drug on cell viability of
tongue cancer cells (SCC-25). IC50 of NPSO_CH/SCC25 was 22.0±0.86 µg/ml while that of
Taxol/SCC25 was 6.28±0.29 µg/ml. Moreover, the caspase 9 enzyme assay revealed a
significant increase of caspase 9 expression values after treatment of cancer cell lines SCC25 with NPSO_CH and Taxol(18.63, 25.94 pg/ml) respectively, in comparison to the
untreated control cancer cells line SCC-25(2.49 pg/ml) .
Conclusion: from this study we concluded that, due to the noticeable increase in the caspase
9 expression caused by NPSO_CH on SCC-25 cell line, NPSO_CH can be used in the future
for chemoprevention, but further investigation and testing is required to validate its anticancer effect.
Keywords: pumpkin seed oil, loaded chitosan nanoparticles, NPSO_CH, Taxol/SCC25.