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العنوان
BIOTECHNOLOGICAL STUDIES ON TOLERANCE OF TARO PLANT TO SOME HEAVY METAL ELEMENTS /
المؤلف
ELSHAWARBY, HALA SOLIMAN MAHMOUD
هيئة الاعداد
باحث / هالة سليمان محمود الشواربى
الموضوع
Vegetable Crops. Agricultural.
تاريخ النشر
2023.
عدد الصفحات
95 p.:
اللغة
الإنجليزية
الدرجة
ماجستير
التخصص
الهندسة الزراعية وعلوم المحاصيل
تاريخ الإجازة
1/1/2023
مكان الإجازة
جامعة بنها - كلية الزراعة - الكيمياء
الفهرس
Only 14 pages are availabe for public view

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Abstract

The present study was carried out during the period from 2016 to 2018 for two parts of the experimental work. The first part was in vitro culture and the second part was in vivo culture, using the Egyptian local taro cultivar (Colocasia esculenta var. esculenta, Schott.).
In vitro experiments were conducted at the plant tissue culture laboratory , Horticulture Department, Faculty of Agriculture, Benha University , to improve number and growth of plantlets in vitro propagation and then to evaluate the field performance of in well adapted taro plants obtained by meristems culture as compared with the traditional propagation methods. Results indicated that leaf pieces induced 90 % of callus, while stem segments formed 80% of callus .The apical meristem and cormal pieces did not induce callus after 2 weeks of culturing on the (Murashige and Skoog) medium supplemented with Benzyl Amino Purine ( BAP) and Naphthalene acetic acid (NAA) and only explants of apical meristem produced (98%)shoots. As for the production of callus, apical meristem formed (zero%), leaves (90%), stems (80%) and cormel (zero %). As for the production of shooting, apical meristem (98%) and the other parts (zero%). These results clearly indicate that among the tested growth regulators types at different concentrations, the apical meristem was the most effective ones to produce the highest number of shoots and leaves explant and the cormel pieces was the least. The treatments BAP (0.20mg/L) and NAA (0.1mg / I) and (40g) sugar gave the highest values of roots number (3.16 and 4.01 roots), respectively .The lowest treatments in this regard were BAP at (0.2 mg/l), NAA at (0.1mg /L) and 30g sugar as well as control treatment which scored (2.74 and 3.02 roots), respectively. The rest treatments gave roots number values between these two extremes. The results illustrated that the best media for acclimatization was peat moss which produced 100% of survival plants. The collected materials during this study were selected to serve two main purpose. The first purpose is study of the heavy metals (Cadmium , Lead and Iron)natural contents of soil ,water and taro plant ,thereby different soil samples from two (0-20 cm and 20-40 cm) layers, Taro Dasheen plant samples , Irrigation and drainage water .samples were collected from (Belbies, Mashtool Al- sook and Mini Elkamh )locations at sharqia governorate.
The second purpose is study the enfluence extansive usage of different fertilization systems,bio waste composte and near bas roads ,on different levels of Cd , Pb and Fe on plant growth.