الفهرس 
IntroductionOnly 14 pages are availabe for public view
 |  | from | 212 |  |  |
| | | from | 212 | | |
Abstract
This study was carried out to obtain a better understanding of the prevalence and molecular
characteristics of Klebsiella pneumoniae (K. pneumoniae (in broiler chicken and humans with assessing its
public health significance in Ismailia city. A total of 531 samples of broiler chickens (481) and human
patients (50) were submitted for bacteriological and molecular examinations for K. pneumoniae. K.
pneumoniae was recovered from 65 out of 481 chicken’s organ samples with an overall isolation rate of 13.5
%. The isolation percentage in broilers was variable according to the organ of isolation. The isolation rate
of K. pneumoniae in human clinical samples was 26%. All thirty-three (20 of broilers and 13 of pateints) of
the previous isolates were positive for16srRNA gene. The antimicrobial sensitivity test for k. pneumoniae
isolates from broiler chickens, showed complete antimicrobial resistance towards ampicillin, lincomycin,
colistin, fosfomycin, trimethoprime/sulphamethaxole. The all isolates were sensitive to imipenem. The
human isolates showed complete antimicrobial resistance towards lincomycin and Fosfomycin. In chicken
isolates, the cmlA, dfrA1, mcr1, tetA, fosA and sul1 resistant genes were detected in 13(65%), 13 (65%) ,12
(60%), 10 (50%),8(40%) and 7 (35%) of isolates, respectively. On the other hand, in human isolates; the
mcr1, cmlA and sul1 genes were detected in 8 (61.5%), 7 (53.8%),6 (46.2%) isolates, respectively. fosA gene
was detected in 3 isolates (23.1%) and tetA in 2 isolates (15.4%), While dfrA1 gene was not detected.
Regarding the virulence gene profiling of K. pneumoniae isolates: In chickens, the rmpA gene was detected
in 17 isolates (85%), either alone in 9 isolates (52.9%) or in combination with the magA gene in 8 isolates
(47.1%) and with fimH gene in 8 isolates (47.1%). The fimH gene was detected in 11 isolates (55%) and the
magA gene was detected in 8 isolates (40%). While in human isolates, fimH gene was detected in 5 isolates
(38.5%) and both rmpA and magA genes were detected each in 6 isolates (46.2%). In chickens, K1 serotype
is more prevalent among hvKP isolates and K2 serotype is rather uncommon in K. pneumoniae isolates. In
conclusion, the current study showed a highly diverse of K. pneumoniae virulence and resistance genes in
chickens and humans with high levels of antimicrobial resistance. K1 serotype is more prevalent among
hvKP chicken isolates. These findings could be a potential threat to food safety and public health.