الفهرس 
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Abstract
The aim of the present study was to evaluate the efficiency of using Hyaluronic acid hydrogel as scaffolds in pulp Regeneration of immature necrotic dogs. The study includes histohologic, and radiographic evaluation.
Animal work was performed for radiographic and histoologic evaluation of the treatment outcomes over 3 months of follow up. The total number of teeth (16) were divided into 4 equal groups according to scaffold group A (Hyaluronic acid), group B (Collagen), group C (Blood clot) and group D (Control). Each group was both radiographically and histologically evaluated.
Induction of infection was done followed by canal disinfection using the calcium hydroxide paste. Treatment protocols were performed according to the previous classification and samples were left for follow up.
Radiographic evaluation included measuring increase in length, thickness and occurrence of apical closure. Radiographs were standardized using Image-J software with TurboReg plug-in. Increases in length and thickness were measured in each group, data were collected and statistically analyzed. Apical closure score was counted and data were statistically analyzed.
Histological evaluation included inflammatory reaction, tissue in-growth, and apical closure. Evaluation of inflammatory reaction included inflammatory cell count for all groups. The tissue ingrowth was quantitatively evaluated using an appropriate scoring system. Data were collected, tabulated, and statistically analyzed.
Results:
Radiographic evaluation showed no significant increase in length and thickness for all groups after 3 months. No significant difference in apical closure was noticed at three months, statistical analysis showed that no significant difference was found between all groups. Also no significant difference was found between groups in dentin thickness.
Histological evaluation of tissue regeneration revealed apparent tissue invasion with cellular elements in the experimental groups more than that in the control group. The inflammatory cells were obviously observed in the control group in the apical periodontal ligament with statistically significant difference. Occasional periodontal damage areas were observed. The apical closure showed no apparent change between the groups.