الفهرس 
IntroductionOnly 14 pages are availabe for public view
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Abstract
Approximately, one million people are affected by head and neck cancers (HNCs) annually, it is the sixth most frequent malignant neoplasm worldwide. One of the most common malignancies of the HNCs is oral squamous cell carcinoma (OSCC). The most prevalent type OSCC is tongue squamous cell carcinoma (TSCC), which is highly invasive and capable of causing extensive and early lymph node metastases One of the traditional forms of cancer treatment is chemotherapy. In addition to relieving symptoms, it also increases the survival and quality of life for patients with incurable cancers. Cisplatin (Cis) is one of the most widely used chemotherapeutic drugs for OSSC that has a strong antitumor effect. However, chemotherapeutic patients may experience severe stress that actually harm their health. Consequently, it is important to find a novel, reliable, and potent cancer treatment, therefore the attention is now directed for producing a safety cancer therapy. Resveratrol (RSV) is a naturally polyphenolic stilbene that have numerous health benefits including, antioxidant, anti-inflammatory, cardioprotective and anticancer activities without genotoxicity. Various mixtures of a chemotherapeutic agent and natural product have been shown to be beneficial in treatment of different types of cancer in the English literature. It has been demonstrated that using Cis together with RSV has synergistic effects on treatment of numerous types of cancer . Accordingly, the aim of this study was to investigate the efficacy of RSV with or without different doses of Cis on the biological activities of human tongue squamous cell carcinoma cell lines; namely the epithelialmesenchymal transition, migration, and viability of such tumor cells. Tongue SCC cell lines (CAL-27) were used. Cis, RSV as interventions agents and anti-Slug antibody as EMT indicator. The TSCC cell line was subcultured to obtain 6 groups and classified as follow: control group (untreated) (A group), RSV group (EXR group), Cis 2 μg/ml group (EXC2 group), Cis 5 μg/ml (EXC5 group), RSV with Cis 2 μg/ml group (EXR/C2 group) and RSV with Cis 5 μg/ml group (EXR/C5 group). The study groups were evaluated using the MTT assay for assessment of the viability of cancer cells and the morphological alterations were assessed by contrast light microscope. However, cell migration was assessed through wound healing assay. Finally, EMT was investigated by assessment of the immunocytochemical Slug expression. The present results revealed that RSV and Cis demonstrated antiproliferative and cytotoxic effects against TSCC cells which were evaluated by MTT assay, it also revealed structural changes that showed by phase contrast inverted light microscope. The wound healing assessment revealed that RSV enhanced the anti-migratory effect of Cis. Furthermore, the ICC of Slug maker expression as EMT indicator, exhibited reduction in the activity of Slug in experimental groups in comparison to control group, with high statistically significant. Statistical analysis of the current results showed a highly significant difference on comparing percentage of viable cells, percentage of migratory cells and levels of Slug expression among all the studied groups. In conclusion, RSV can sensitize the effects of Cis. This suggested that to achieve the best benefits with less side effects, it is advised to add RSV with low dose of Cis.