الفهرس 
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Abstract
SUMMARY
This study aimed to investgate the genomic comparison and the molecular genetic similarities between Lumpy Skin Disease Virus LSDV and Sheep pox virus SPV.
Which Lumpy Skin Disease Virus spread widely in Egypt with a purpose to use Sheep pox vaccine to vaccinated cattle which is infected with Lumpy Skin Disease virus . This is to identify them by propagation of LSDV on MDBK cell culture and propagation of SPV on VERO cell culture whrere watch the cell aggregation, rounding, degradation and vaculation after five dayes. Then titrated the viruses to khnow the ratio of virus to the cellwhich infected with it.
On the other hand, compare between two viruses by using Polymerase Chain Reaction PCR analysis by using G-protein-Coupled Chemokine receptor (GPCR) specific primer for Capripoxviruses and another specific primer a viral protein gene (P32) primer, through Molecular weight of viruses and comparison by the nucleotide sequence and amino acid of Sheep pox isolates and a virulent sheep pox (Egyptian sheeppox virus) and vaccinal strains (Romanian sheep pox virus and Kenyan sheep pox virus) which obtained high semelarity with Lumpy skin disease virus, these results give the reason to know the immunity of cattle when vaccinated with sheep pox vaccine rather than Lumpy skin disease vaccine (Traditional vaccine).
In this study we isolated and characterized through molecular genetics of both LSDV and SPV in purpose to know the immunogenic relationship between LSDV and SPV leading to protection via the following steps:
1- Collection of skin lesions samples from cattle showing LSD-and Sheep SPV.
2- Centrifugation until obtained about pure virus.
3- Propagation of the isolated virus was passaged 5 passages on the MDBK for LSDV .
4- Propagate sheep pox virus on Vero cell culture until CPE which appear rounding, aggregation, degradation and vaculation.
5- Titrated of Lumpy skin disease virus on MDBK which found to be 106.32 Tissue Culture Infected Dose TCID50/ml. and was titrated sheep pox virus 106 TCID50/ml.
6- Antigenic characterization by Molecular Comparison between thim by:-
7- Extraction of the DNA of Lumpy skin disease virus LSDV and Sheep pox virus SPV was performed .
8- Amplification was done by PCR for a genome of LSDV and SPV by using specific primers GPCR primer and P32 primer the product was sequenced by using automated Sequencer for purified fragment.
9- Studies the alignment of amino acid and nucleotide sequence of partial P32 and GPCR gene.
10- Phylogenetic analysis using the partial sequences of the amplified genes was done The analysis of the obtained sequences and the antigenic characterization revealed the high degree of relatedness between LSD and SPV the sequence analysis as well as antigenic characterization demonstrated high degree of relatedness between two viruses as revealed after sequencing and phylogenetic tree that we can vaccinated cattle with Sheep pox Vaccine.