الفهرس 
IntroductionOnly 14 pages are availabe for public view
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Abstract
Primary angle-closure glaucoma is a neurodegenerative
disease characterized by elevated IOP due to a mechanical
obstruction of the TM either by apposition of the peripheral
iris to the TM or a synechia closed angle. PACG is a complex
heterogeneous disease with multifactorial causes that can
affect the progression of the anterior chamber angle from
narrow to angle-closure. These factors include genetic factors
and oxidative stress. The current study aimed to investigate the
associations between PACG and rs11024102 in PLEKHA7,
rs3753841 in COL11A1, and the systemic oxidative stress
markers in Egyptian patients.
This case-control study enrolled 35 control subjects and
64 PACG patients. The polymorphisms in PLEKHA7 and
COL11A1 were analyzed using qRT-PCR followed by
statistical analysis for their associations with PACG. In
addition, the serum levels of MDA, AOPP, PC, and IMA
(oxidative stress biomarkers) were quantitated
colorimetrically. Finally, the associations of the oxidative
stress biomarkers with both PACG and elevated IOP were
statistically analyzed.
The results of the current study showed that neither
significant difference in the genotype distribution nor allele
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frequency of PLEKHA7 11024102 T>C (p > 0.05) and
COL11A1 rs3753841 G>A (p > 0.05) are recorded under any
of the tested genetic models (homozygous, heterozygous,
dominant, and recessive). Moreover, both rs11024102
PLEKHA7 and rs3753841 COL11A1 did not show a significant
association with the increased risk of PACG (p > 0.025 after
Bonferroni correction) in Egyptian patients.
Patients with PACG showed significant elevations in the
serum levels of MDA, AOPP, and PC either in patients with or
without diabetes mellites, hypertension, CVD, and smoking.
On the other hand, the serum level of IMA showed no
statistically significant change (p > 0.05) in PACG patients. Of
these oxidative stress biomarkers, MDA, AOPP, and PC were
significantly associated with PACG in Egyptians (p < 0.013
after Bonferroni correction).
Multiple linear regression analysis showed a significant
correlation between the serum level of MDA and the elevation
in the IOP in PACG patients (p = 0.005) (n=64) including
cases with DM, hypertension, CVD, and smoking.
Surprisingly, both MDA and PC levels showed significant
correlations with the elevated IOP (p = 0.007 and 0.045,
respectively) after excluding the cases of DM, hypertension,
CVD, and smoking (n = 36).
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Interestingly, an independent T-test performed to explore the
effect of using the intraocular medications on the correlations
of the oxidative stress biomarkers with the elevated IOP
reports that the serum level of MDA showed a more elevation
in PACG patients under intraocular medications (n=34) than
patients who did not take any intraocular medications (n=2) (T
= -2.33, p = 0.033). Moreover, the serum level of IMA showed
a more reduction in patients under the intraocular medication
(T = 2.91, p = 0.006).
In conclusion, PLEKHA7 rs11024102 T>C and
COL11A1 rs3753841 G>A SNPs could not be considered risk
factors for PACG in Egyptians. However, systemic oxidative
stress (the elevating serum levels of MDA, AOPP, and PC)
was found to be a probable risk factor for PACG. Of these
markers, only serum levels of MDA and PC were considered
significant predictors for the elevation in the IOP average.