الفهرس 
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Abstract
Canine parvovirus-2 (CPV-2) and canine coronavirus (CCoV) are two viruses responsible for
the most common causes of diarrhea in dogs. This study aimed to investigate the risk factors for
CPV-2 and CCoV infections in diarrhetic dogs in Ismailia governorate, Egypt. Furthermore, both
viruses were molecularly characterized. from September 2021 to July 2022 a total of 150 fecal
samples were collected from bloody diarrhetic dogs visited Veterinary Clinics. About 149 out
of 150 (99.3%) and 13 out of 150 (8.7%) were positive for CPV-2 using conventional PCR and
CCoV using our newly developed one-step probe based quantitative RT-PCR (qRT-PCR),
respectively. All the positive samples for CCoV were tested by conventional RT-PCR for S gene
amplification. Only one sample was positive and further sequencing and phylogenetic analysis
confirmed detection of CCoV I genotype. To our knowledge this is the first study confirmed
circulation of this strain in Egypt. On the other hand, Sequencing, and molecular analysis of 15
CPV-2 positive samples revealed detection of CPV-2c. We correlate between percentage of
positivity and different variables. In our study, more CPV-2 positive cases were reported in
April, May, and June. More clinical cases of canine parvovirus were reported from male dogs
(62.4%), from German shepherd breed (31.5%) and from non-vaccinated dogs (71.8%). On the
other hand, the risk factors analysis revealed significant association between Pitbull dog breed
and infection by CCoV.