الفهرس 
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Abstract
The present work was performed to study the Molecular mechanism underlying the protective role of anthocyanins on UV-radiation induced DNA damage in HepG2 cells. We have shown that Hibiscus sabdariffa anthocyanins, significantly reduced DNA damage in UV irradiated HepG2 cells and that this protection of DNA is related to autophagy induction.
The main results obtained from this study are summarized as the follow:
• There was approximately no effect on HepG2 cell viability at a concentration of 0.5 μg/ml anthocyanins. Therefore, 0.5 μg/ml anth. was used in all experiments to avoid cell death while also protecting cells from damage caused by UVB radiation.
• Anthocyanins promotes autophagy induction in HepG2 cells.
• Anthocyanins protect HepG2 cells against UVB-induced DNA damage.
• Inhibition of anth.-induced autophagy reverses its protective effect against UVB-induced DNA damage.
• Autophagy induction by anth. is the key player in the
protection role of cells against UVB-induced DNA damage.
• Autophagy mediates the protection effect of anth. Against UVB-induced DNA-protein crosslinks formation.
• Autophagy mediates the protection effect of anth. Against UVB-induced cyclobutane pyrimidine dimers and thymine dimer formation.
• Autophagy induced by anth. controls p53 transcription and its p21 transactivated protein.