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العنوان
Prevalence of Shiga Toxin Producing Escherichia coli among Dogs and Cats /
المؤلف
Abotalp, Eman Hesham.
هيئة الاعداد
باحث / إيمان هشام ابوطالب
مشرف / جاكين كمال عبد الحليم الجاكى
مشرف / سحر رشدي محمد
الموضوع
Dogs. Cats. Multidrug resistance. Diarrhea. E. coli infections.
تاريخ النشر
2023.
عدد الصفحات
63 P. :
اللغة
الإنجليزية
الدرجة
الدكتوراه
التخصص
البيطري
تاريخ الإجازة
1/1/2023
مكان الإجازة
جامعة القاهرة - كلية الطب البيطري - Microbiology
الفهرس
Only 14 pages are availabe for public view

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from 84

Abstract

Escherichia coli (E. coli) are intestinal bacteria that affect people and animals like dogs and cats. Pets are normally in close contact with their owners, and so harmful microorganisms can be easily transmitted from them to human beings. So, the ongoing study was applied to be aware assuming dogs and cats in Egypt are colonized with unsafe E. coli serogroups, it`s antimicrobial resistance, and it`s virulence genes. A total of 129 rectal swabs were gathered from apparent healthy and diarrheic dogs and cats. By using Vitek2 compact system, 42 E. coli isolates (32.6%) were resulted from dogs (24%) and cats (44.4%) rectal swabs. E. coli were serogrouped to: O8, O25, O26, O28, O36, O55, O78, O86, O111, O114, O125, O127, O128 and O157. The resistance to 16 antimicrobial and the creation of extended-spectrum β-lactamases (ESBLs) were identified on E. coli isolates by using Vitek2 compact system. The creation of ESBL was distinguished in 5 of the isolated E. coli. The highest antimicrobials resistance was toward ampicillin (60%) and trimethoprim sulfamethoxazole (45%). No resistance was observed to piperacillin/tazobactam, meropenem and amikacin. On examination of 20 isolates of E. coli by PCR for the presence of virulence genes (stx1, stx2, eaeA, and hlyA) resulting to 2 (10%), 3 (15%), 6 (30%), and 15 (75%) were positive for stx1, stx2, eaeA, and hlyA, respectively.