الفهرس 
Staphylococcus aureus
Immune chromatographic test (Latex agglutination)Only 14 pages are availabe for public view
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Abstract
Summary Antimicrobial resistance is increasing rapidly worldwide, becoming one of the leading global threats to public health. Methicillin resistant Staphylococcus aureus (MRSA) has been recognized as a common cause of healthcare-associated infections (HAIs) leading to increased mortality, longer hospitalizations and higher costs to healthcare systems.Rapid detection of MRSA is crucial for early treatment of patients and performance of infection control approaches. There are many phenotypic and molecular methods for detection of Methicillin resistance in (S. aureus). In most cases, phenotypic methods are easier than molecular methods, while molecular methods may have better accuracy, precision and remain the reference standard for the identification of MRSA.Since the past two decades, Loop mediated isothermal amplification (LAMP) has gained enormous popularity as a rapid and cost-effective detection method for numerous infectious diseases. LAMP is a low-cost diagnostic as the method is isothermal, there is no need for expensive thermocyclers to regulate the temperature as with PCR; only a simple heating block or water bath is required. LAMP is considered a simple-tooperate and easy-to-read molecular diagnostic tool for both laboratory and on-field settings.
So, the aim of this study was to identify MRSA among strains isolated from Medical and Surgical Intensive Care Units (ICUs) of Tanta University Hospitals by two phenotypic methods, determine susceptibility of MRSA isolates to different antibiotics, detect MRSA genes mecA and femB by LAMP assay and Real-time PCR and evaluate sensitivity and specificity of LAMP assay for rapid detection of MRSA