الفهرس 
Title : اTherapeutic and immunological studies on parasitic gastroenteritis in ruminants
AbstractOnly 14 pages are availabe for public view
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Abstract
Parasitic gastroenteritis (PGE) is a disease complex affecting ruminants and lead to greateconomic losses including reduction in the productivity, stunted growth, reduced weight gain, low fertility, high treatment costs, and mortality. The aim of this thesis is tocarry out epidemiological surveillance of parasitic gastroenteritis affecting ruminants in Egypt, to do comparative evaluation between traditional anthelmintics and new natural and synthetic anthelmintics against gastrointestinal nematodes both in vivo and in vitro and to evaluate the immunological effect of the prepared somatic antigen of nematode ( Haemonchus spp.) in laboratory animal.a total of 120 ruminants of different species were examined for PGE eggs in their fecal samples and were divided into four groups to compare between Pomegranate peel extract and synthetic drugs and results showed an increase in FEC in the control group by a 10% and 100% reduction in FEC in synthetic anthelmintics treated groups. While in PPE treated group, there was a decrease in FEC by 97%. In conclusion, PPE could be usedas a safe, cheap, and effective natural anthelmintic against PGE.silver nanoparticles (AgNP), selenium nanoparticles (SeNP), and (Punica granatum) pomegranate peel extract (PPE) were used in vitro to evaluate their anthelmintic effect on Haemonchus contortus different stages (eggs, larvae, and adults) using EHA, LPA and WMI testes.It was found that AgNP, SeNP, and PPE induce inhibition of egg hatching. Also, AgNP, SeNP and PPE induced larvicidal activity. AgNP, SeNP and PPE induced a significant adulticidal activity compared to control.Whole adult soluble extract of Haemonchus contortus as an antigen along with Nigella sativa oil adjuvant, were used to immunize rabbits. Sera samples were collected from immunized rabbits at different intervals of 0,14, 21, 28, 35, 42 and 49 days post immunization. For the detection and titration of antibodies against H.contortus in these sera, ELISA was developed.