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Abstract
Fungi are considered as the second largest group after insects, and widely distributed in nature, they are known for their capacity to secrete high levels secondary metabolites. The study is concerned on the chemical and biological evaluation of Trichoderma viride (Family Moniliaceae) and Chaetomium globosum (Family Chaetomiaceae). The air-dried powders of the mycelia of both fungi subjected to phytochemical screening and proximate analysis. The crude extracts, volatile constituents, different successive fractions of the mycelia and the liquid fungal filtrate were prepared and the yields, physical and chemical characters were determined. Both T. viride and C. globosum exerted great antifungal effect and significant antioxidant activity. The volatile constituents of the fresh T. viride and C. globosum mycelia were 0.19% and 0.2% respectively. Thirty compounds have been identified (78.25%) of T. viride mycelia, while Thirty two compounds have been identified (80.87%) of the mycelia of C. globosum. The total sterols in the crude extracts of T. viride and C. globosum mycelia are 13.95% and 14.75% respectively, while total terpenes are 38.34% and 40.53% respectively. The estimation of protein in the crude extracts of T. viride and C. globosum are 10% and 20% respectively. Isolation of protein gave a percentage of 8.5% and 17.8% respectively. HPLC analysis of the free amino acid content were performed for both fungi. The total carbohydrate content in the dried crude extract of T. viride and C. globosum mycelia were 19.57% and 25.14% respectively. PC and HPLC analysis of the mucilage hydrolysate were performed for both fungi.