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Abstract
Seventy three isolates of AIV were collected from 11 Egyptian governorates during the period from 2010 to 2014, from different poultry flocks, The clinical signs were cyanosis of comb and wattle, echymosis on the shanks and feet, facial edema and greenish diarrhea and DROP in egg production .On necropsy, diffuse subcutaneous hemorrhages on the feet and shanks, the lung showed congestion and hemorrhages. Petechiae were noticed throughout on serosal surfaces especially on the peritoneum. The ovaries were hemorrhagic contains yolk from ruptured ova. RT-PCR revealed 43 positive for M gene while 26 positive using H gene primer whereas cleaveage site sequence revealed 17 out 73 positive with percentage 58.9%, 35.6% and 23.2%; respectively. AI strain (A/chicken/Egypt/M7217B/2013(H5N1) represent the most common clusters of AIV was reassorted at National research center and then adapted on MDCK cell line for preparation of Tissue culture (TC) vaccine in parallel with egg adapted one