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العنوان
Enhancement of azo dyes biodegradation by bioaugmentation and immobilization of microbial cells in wastewaters /
الناشر
Walaa Ahmed Ameen Alshareef ,
المؤلف
Walaa Ahmed Ameen Alshareef
هيئة الاعداد
باحث / Walaa Ahmed Ameen Alshareef
مشرف / Mohammad A. Ramadan
مشرف / Abdelgawad M. Hashem
مشرف / Tamer Essam
تاريخ النشر
2014
عدد الصفحات
165 P. :
اللغة
الإنجليزية
الدرجة
الدكتوراه
التخصص
العلوم الصيدلية
تاريخ الإجازة
1/4/2015
مكان الإجازة
جامعة القاهرة - كلية الصيدلة - Microbiology and Immunology
الفهرس
Only 14 pages are availabe for public view

from 198

from 198

Abstract

Biological decolorization of dyeeffluent is receiving much consideration due to cost effective and its safety use. A total of 35 isolates (8 bacteria and 17 fungi) were recovered from waste waters by the enrichment culture technique, using Reactive black 5 (RB5) as a sole carbon source. A bacterial isolate (identified as Micrococcus luteus) showed the highest biodegradation activity. This isolate was able to remove up to 66% of the dye within 3 days of incubation. Comparatively, 53 % of the dye was decolourized by a yeast isolate (identified as Candida albicans) after 3 days of incubation. Addition of yeast extract at a concentration of 10 g l-1 enhanced the biodegradation, where complete disappearance of the dye was observed within 3 days of incubation. Interestingly, the bacterium was able to decolorize the dye up to a concentration of 40 mg l-1. The residual of the dye after degradation, was traced, where a metabolite was detected by TLC, UV absorbance as well as HPLC after 5 days of incubation by Micrococcus luteus. The metabolite was detected by TLC with Rf= 0.8 and a wavelength of 597 nm