الفهرس 
KLEBSIELLA PNEUMONIAEOnly 14 pages are availabe for public view
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Abstract
Klebsiella pneumoniae is considered as a common cause of both hospital-acquired and community-acquired infections. It is characterized by the presence of different virulence factors, the most important one is biofilm formation and also this organism is characterized by the emergence of antimicrobial resistance and most of isolates nowadays are multidrug-resistant organisms which is considered as a challenge for physicians, so researchers try to find a solution for this problem. One of these solutions is the synthesis of nanoparticles that could be used for treatment like silver nanoparticles.
Silver nanoparticles have various effects like antibacterial, antifungal, antiviral, and anticancer action. Also, compared to physical and chemical mechanisms, AgNP’s green biosynthesis is a safer, eco-friendly, and more economical process.
This study was conducted on fifty non-duplicated MDR Klebsiella pneumoniae isolates collected from different clinical samples from the main Microbiology Laboratory of Ain Shams University Hospitals in the period from January to August 2022.
All isolates were subjected to the conventional microbiological identification by using Gram staining, colonial morphology, and biochemical reactions
The distribution of biofilm-forming ability among isolates was as follows: 32% of isolates were moderate biofilm producers while 24% of isolates were weak biofilm producers.
The Silver nanoparticles were synthesized biologically by using aqueous Eucalyptus camaldulensis (E. camaldulensis) leaf extracts and characterized by Ultraviolet-visible spectroscopy (UV-Vis), Transmission electron microscopy (TEM), Fourier-transform infrared spectroscopy (FTIR), and X-ray diffraction (XRD). The antibacterial and antibiofilm activities of biosynthesized AgNPs were investigated against selected strains using the standard method.
Biosynthesis of silver nanoparticles using plant extract exhibits antimicrobial effect with MIC ranging from 15.625 μg /ml to 125 μg /ml and inhibitory conc. to 90% of the isolates (MIC90) 62.5 μg /ml.
Silver nanoparticles significantly reduced the biofilm formation by K. pneumoniae isolates. The mean (±SD) of OD was decreased from 0.5617( ±0.1266) to 0.1190 (±0.0683).
There was a statistically significant relationship between K. pneumoniae isolates and susceptibility results of biologically synthesized silver nanoparticles.