الفهرس 
DIABETIC KIDNEY DISEASEOnly 14 pages are availabe for public view
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Abstract
Abstract
Introduction: Diabetic nephropathy is the most common long-term complication of diabetes mellitus and one of its major complications is diabetic kidney disease which is a major cause of end-stage renal disease (ESRD).Recent studies suggested defective autophagy involved in the pathogenesis and progression of diabetic kidney disease. So, we aimed to assess the autophagy biomarker Beclin-1 level in diabetic kidney disease patients.
Methods: In a cross sectional study , we enrolled 100 patients with Diabetic Kidney Disease (DKD) referred to Ain-Shams University hospital. The patients were divided into 5 groups according to the estimated glomerular filtration rate (eGFR). Each group consisted of 20 patients to Compare the level of serum Beclin-1 in different groups.
Beclin 1 assay: Human Beclin 1 (BECN1) ELISA kit uses a double antibody sandwich enzyme linked immunosorbent assay (ELISA) to assay the level of Human Beclin 1 (BECN1) in samples.
Results:
There was statistically significant difference between the studied groups (p<0.001) regarding hemoglobin, creatinine, hemoglobin A1c, calcium, phosphate, protein creatinine ratio, eGFR, and Beclin-1 levels with highest value among group (2, 5, 4, 2, 5, 5, 5 and 5) respectively and lowest value among group (5, 1, 5, 5, 2, 1, 5 and 5) respectively. There was statistically significant inverse correlation between Beclin- 1 and diabetes duration, creatinine, urea, CRP and protein creatinine ratio (r: -0.24; p= 0.026), (r: -0.44; p< 0.001), (r: -0.44; p<0.001), (r: -0.52; p<0.001), (r: -0.56; p<0.001) respectively. There was statistically significant positive correlation between Beclin- 1 and hemoglobin, calcium and eGFR (r: 0.242; p= 0.015), (r: 0.248; p= 0.013) and (r: 0.568; p<0.001) respectively. At cutoff value equal to (3.3, 1.2, 1.8 and 1.1) Beclin- 1 had sensitivity equal to (100%, 55%, 80% and 85%) respectively and specificity equal to (85%, 85%, 75% and 100%) respectively in prediction of CKD progression from (stage 1 to stage 2), (stage 2 to stage 3), (stage 3 to stage 4) and (stage 4 to stage 5) respectively.
Conclusion: In conclusion we found that eGFR was the most influential parameter affecting Beclin-1 level and by using multivariate model, linear regression analysis adjusted factors for confounders eGFR was the only factor which kept his significant correlation with Beclin- 1.