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العنوان
Rapid Detection of Carbapenemases Producing Enterobacteriaceae (CPE) By chromatic TM CPE Media in Intensive Care Units in Ain Shams University Hospitals /
المؤلف
Elshenawy, Amira Gaber Soliman.
هيئة الاعداد
باحث / أميرة جابر سليمان الشناوى
مشرف / شيرين بندارى السيد بندارى
مشرف / فاطمة مصطفى محمود
مشرف / ياسمين محمد محمود علي
تاريخ النشر
2023.
عدد الصفحات
134 p. :
اللغة
الإنجليزية
الدرجة
ماجستير
التخصص
علم الأحياء الدقيقة (الطبية)
تاريخ الإجازة
1/1/2023
مكان الإجازة
جامعة عين شمس - كلية الطب - الميكروبيولوجيا الطبية والمناعة
الفهرس
Only 14 pages are availabe for public view

from 134

from 134

Abstract

Early detection for ICU infection caused by carbapenem resistance Enterobacteriaceae (CRE) can guide rapid life-saving appropriate therapy. A new variant of chromatic TM CRE medium can detect CRE directly from the clinical samples.
This study aims comparing the samples detected by chromatic TM CPE medium in identifying CREs directly from ICU clinical samples to mCIM as standard test for identification of CREs.
One hundred and fifty-eight isolates were obtained from hospitalized ICU patient in Ain shams university hospital between. 158 patients with different types of samples the most common type was sputum by 69% followed by wound swabs by 23.3%.
Out of 158 isolates, 87 (55.1%) showed no growth on blood and MacConkey agar media while 71 (44.9%) showed growth of gram-negative isolates. Among 71 isolates grew on blood and MacConkey agar media, 59 (83.15%) showed growth on chromogenic media while 12 (16.9) showed no growth on chromogenic media.
According to the result of antimicrobial susceptibility test done according to CLSI 2022, out 71 isolates, 17 (23.9%) showed normal flora or isolates sensitive to carbapenems while 54 (76.1%) showed MDR Enterobacteriaceae, Pseudomonas aeruginosa and Acinetobacter baumannii. Distribution of CRE isolates was Klebsiella pneumonia 30 (42.3%).
Detection of carbapenemase producing Gram negative bacilli, shows a fair agreement (94.4%) in all Gram-negative bacilli isolates as (kappa value = 0.542) when comparing the results of the chromogenic media to the mCIM, and poor agreement (91.7%) in case of Enterobacteriaceae as (kappa value = 0.357) and excellent agreement (100%) (kappa value = 1.00) in Pseudomonas aeruginosa. The application of kappa agreement measures in Acinetobacter baumannii was not applicable as there were no negative cases in mCIM.
The sensitivity of chromogenic media to detect true positive results in all isolates was 98%, specificity to detect true negative results was 50% and the accuracy of the test was 94.4%. While the sensitivity of chromogenic media to detect true positive results in all Enterobacteriaceae was 96.9%, specificity to detect true negative results was 33.3%.