الفهرس 
ACKNOWLEDGEMENTOnly 14 pages are availabe for public view
 |  | from | 93 |  |  |
| | | from | 93 | | |
Abstract
Milk, due to its high nutritive value; is considered an excellent medium for the growth of pathogenic as well as non-pathogenic bacteria. This may lead to milk borne outbreaks caused by different types of bacteria such as Coliforms, E.coli, Enterobacter spp. And Cronobacter sakazakii .
A total of 150 random samples of milk including farmer’s houses, dairy farms and dairy shops (50 samples for each) have been collected from different localities in New Valley Governorate, Egypt. The samples were microbiologically examined, and isolates were identified by biochemical, serological and PCR for the presence of Coliforms, E.coli, Enterobacter spp. Cronobacter sakazakii .
Overall the prevalence of Coliforms was 13.3% of the all examined milk samples. The prevelance of Coliforms was 20, 6 and 14% from farmer’s houses, dairy farms and dairy shops milk samples, respectively.
These results revealed that the examined milk samples were contaminated with Coliforms with an average counts of 1.9x104, 6.6 x104 and 4.0 x104 cfu/ml, from farmer’s houses, dairy farms and dairy shops, respectively.
The prevalence of E.coli in the total examined milk samples was 2.66% and in farmer’s houses, dairy farms and dairy shops was 2, 2 and 4%, respectively. Serologically results of E.coli isolates were O127K63 from farmer’s houses, O55 from dairy farms and O114K90 , O44K74 from dairy shops, (monoclonal isolates, EPEC pathotype.)
Furthermore, only two strains of isolated E.coli carried eaeA virulence gene. On the other side, all the isolated strains of E.coli didn’t carry hly A gene.
In addition, the prevalence of Enterobacter spp. In the total examined milk samples was 3.33% and from farmer’s houses, dairy farms and dairy shops milk samples was 4, 4 and 2% , respectively.
These results also revealed that the examined milk samples were contaminated with C. sakazakii with percentage 14 and 8% from farmer’s houses, dairy shops milk samples, respectively. While prevalence in total examined milk samples was 7.33%. And it could not be detected from dairy farms milk samples.
The obtained results of present study proved that farmer’s houses milk samples more contaminated with coliforms and C.sakazakii than dairy shops and dairy farms. While dairy shops milk samples more contaminated with E.coli than other, also obtained data indicated that C.sakazakii could not be isolated from dairy farms milk samples. Concerning public health importance of these pathogens that affect human health through consumption of milk were discussed. Likewise, the suggestive measures for improving the quality of milk.