الفهرس 
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Abstract
The enzymes of Bacillus circulens and Bacillus coaculens were used in the production of copper and silica in nanoforms, respectively, by evaluating several treatments on each compound to convert the compounds from the normal form to the nanoform due to the action of the enzymes of the bacteria used. The source of copper used is aqueous copper sulfate and the source of silica is silicate Sodium. A liquid sample and a dry sample were taken from each compound according to the best results obtained for conducting TEM and XRD nanomaterials tests. Laboratory tests were carried out on some pathogens that cause root rot in sugar beet crop, namely, Fusaruom oxysporium, Microphomena phasolina and Bacterobacterium carotovorum grown in Petri dishes under laboratory conditions for a week as a comparison between CuO-NPs and copper in the normal form. Another experiment was conducted in plastic pots for a month, and the results were compared with the natural and infective control with the pathogen not treated with copper seeds, where the concentration of µmL-1150 gave the best result in resisting the pathogens of the plant and not infecting it completely. A greenhouse experiment was conducted for a period of 3 months. The following concentrations were used (control without infection, control with infection without treatment, seed treatment with Vitavax, a commercial product, and concentrations of 50, 100, and 150 µmL-1. The most important results obtained were:
The concentration of 150 µmL-1 gave the best results with the used pathogens, as it gave an infection rate of 0% and the highest values with all the measurements that were made on the soil and the plant (root and leaves) such as chlorophyll a and b, total chromophyll, carotene, total soluble sugars, measurements of nitrogen, phosphorus, potassium and copper concentration Some antioxidant enzymes such as (POX) peroxidase enzyme, (PPO) polyphenol oxidase, (PAL) phenylalanine lyase were measured, while the infective control with the pathogen without seed treatment was given the highest infection rate of 100% and the lowest values with all measurements. from the study we find CuO- NPs gave significant positive results with pathogens that cause root rot in bengal sugar crop, and it is recommended to apply them to the agro-environmental field more widely.