الفهرس 
Rapid diagnosis of mastitis caused by mycoplasma species in dairy cows using polymerase chain reaction
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Abstract
Mycoplasma species are highly contagio us patho genscause a serious problem on dairy farms. The present study aimed to detect the incidence of Mycoplasma mastitis and the causative species of infection , and to compare between conventional methods and multiplex PCR for detection of M. bovisand M. bovigenitalium directly from the examined milk samples. A total of 956 milk samples from cows suffered from mastitis as well as 13 milk tank samples were investigated. Then a total of 50 randomly selected samples from the mentioned individual cow ’s milk as well as 5 from milk tanks were subjected to comparative study between conventional and multiplex PCR . Multiplex PCR was performed for identification of M .bovisand M. bovigenitaliumusing specific primers. Also In this study 2Mycoplasma bovis (accession no . KU90073 1 and KU900732) isolates and 2 Mycoplasma bovigenitalium (accession no . KU900733 and KU900734) were analyzed .The results revealed that the m PCR matched the conventional method among the investigated samples except in : Farm 7 whereas the m PCR detected three M. boviswhile the conventional method d could detect one. Farm 5 and farm 9 whereas both M. bovis and M. bovigenitalium were detected in 4 and 2 samples respectively. It could be concluded that PCR -based technology for Mycoplasma yields the highest level of sensitivity and specificity