الفهرس يوجد فقط 14 صفحة متاحة للعرض العام
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المستخلص
Streptococcus pyogenes (group A Streptocci-GAS) is one of the most frequent pathogens of humans. It is estimated that between 5-15% of normal individuals harbor the bacterium, usually in the respiratory tract, without signs of disease. As normal flora, S. pyogenes can infect when defenses are compromised or when the organisms are able to penetrate the constitutive defenses. In the last century, infections by S. pyogenes claimed many lives especially since the organism was the most important cause of puerperal fever, Scarlet fever, streptococcal pharyngitis (strep throat). Patients may also develop immune-mediated post-streptococcal sequelae, such as acute rheumatic fever and acute glomerulonephritis, following acute infections caused by Streptococcus pyogenes. 440 quarter milk samples(QMS) from apparently healthy animals (368 from cows72 and from buffaloes), 72 quarter milk samples from clinically infected animals (60from cows and 12 from buffaloes). S. pyogenes was isolated in percentage of (17%) from examined quarter milk samples. By applying Antistreptolysin o titre (A.S.O.T) and Carbohydrate like reacting protein (C.R.P) on Blood samples collected from human cases found to have +ve ß- hemolytic isolates. Out of 35 samples 25 (71.43%) founded to be positive to A.S.O.T and 28(80%) founded to be positive to C.R.P. (100%) of the examined S. pyogenes isolates from bovine milk and human cases were sensitive to bacitracin and Penicillin G. Only14 S. pyogenes strains were selected and subjected for identification using PCR. It was clear from the obtained results that only five (35, 71%) strains were confirmed to be S. pyogenes. 35 strains isolated from throat swab samples proved to be S. pyogenes on using ordinary conventional methods for identification. Only 10 S. pyogenes strains were selected and subjected for identification using PCR. It was clear from the obtained results that only five (50.00%) strains were confirmed to be S. pyogenes