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Abstract
The present study isolated and culture human dental pulp stem cells (HDPSc) and compared the effect of different irrigation solutions including Morinda Citrifolia(MC) as a natural irrigant on the HDPSc attachment to root canal dentine using Electron microscope. Ten human third molars collected for isolation of dental pulp stem cell from 8 healthy medically free young patients age from 17-25 years and twenty five intact human first lower premolars were collected for addition of dental pulp stem cells. After the 4th passage of DPSCs, human bone morphogenic protein type II (rh-BMP 2) was added to Confluent cultures .The cells were added to the cleaned and shaped root canals of the five groups. group I irrigation was done using 5.25 % NaOCl, group II irrigation and final rinse was done using 5.25 % NaOCl and 17%EDTA was used as a chelating agent, group III irrigation was done using MC, group IV irrigation and final rinse was done using MC and17%EDTA was used as a chelating agent, group V (control) Saline was used as irrigation. After 14 days of cell culture, the teeth were removed and processed for scanning electron microscopy. Data were presented as mean and standard deviation (SD) values. Numbers of cells data were compared using non-parametric tests