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العنوان
Biochemical and Molecular Impacts of New Control Measures for Rhynchophorus sp /
المؤلف
Deriza, Basma Mohamed Gabr Mohamed.
هيئة الاعداد
باحث / Basma Mohamed Gabr Mohamed Deriza
مشرف / Mohammed M. El-Bokl
مشرف / Abd Al-Aziz A. Khider
مشرف / Michael J. Adang
الموضوع
Rhynchophorus cruentatus.
تاريخ النشر
2022.
عدد الصفحات
17 P. :
اللغة
الإنجليزية
الدرجة
الدكتوراه
التخصص
علم الحيوان والطب البيطري
الناشر
تاريخ الإجازة
7/5/2022
مكان الإجازة
جامعة دمياط - كلية العلوم - علم الحيوان
الفهرس
Only 14 pages are availabe for public view

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Abstract

The genus Rhynchophorus (Coleoptera: Curculionidae) is considered to have at least ten known weevils species that are significant pests of palm trees. Control of the genus Rhynchophorus is very difficult due to its hidden habitat. While chemical insecticides provide the most popular strategy for controlling palm weevils, their efficacy is challenged by insect resistance and environmental concerns. New management strategies should be developed as a substitute for using chemical pesticides.
Therefore, the current study focused on two important candidates from Rhynchophorus sp.: R. ferrugineus and R. cruentatus. The study is divided into 3 main parts: Gut Transcriptomic study of R. ferrugineus, Rearing experiments, and Laboratory bioassay against R. cruentatus. These investigations aimed to achieve the following purposes:
The Gut Transcriptomic study aimed to report for the first time a full gut transcriptomic profile for both larva and adult of RPW (R. ferrugineus) using long-read data generated from PacBio SMART sequencing and identifying important genes involved in digestion, detoxification, insecticide resistance, and insecticide receptors in both larva and adult females of RPW.
Rearing experiments aimed to establish and maintain a colony of Palmetto weevils (R. cruentatus) under laboratory conditions. This will provide enough different life stages needed for further experiments and bioassays.
Laboratory bioassays aimed to detect, for the first time, efficient methods for controlling Palmetto weevil’s (R. cruentatus) larva depending on naturally occurring products with non-hazard effects on humans such as Neem oil extracts, Spider venom peptide, and Bacillus thurigiensis kurstaki and determine the toxicological activities and mean lethal concentration (LC50) of selected insecticides against larva of palmetto weevil. The impact of selected control methods on feeding behavior and detecting larval weight changes were also reported. We observed the impact of selected control methods against larva of palmetto weevils at biochemical and molecular levels by tracing the toxic action of these selected insecticides on the activity and the gene expression of some detoxification enzymes.
Data revealed in this study would contribute to understanding the impact of some natural and biological insecticides on them which could help in developing the integrated management of Rhynchophorus sp.