الفهرس 
Biomanagement of potato wilt disease using innovative biocontrol formulations
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Abstract
Potato crop is economically necessary for Egypt and any disturbance in its manufacturing impacts over severely affected.As its infected withbacterial wilt (potato brown rot) cause losses of a lot of million worldwide.Therefor is necessary to evaluate the ability of some strains of plant growth-promoting rhizobacteria (PGPR) to control the pathogenicity ofRalstoniasolanacearum in Laboratory and greenhouse experiments. These experiments were conducted at the Potato Brown Rot Project (PBRP), Giza, Egypt. Isolates of pathogenic bacteria collected from three Egyptian governments (Menofia, Gharbia, and Beheria) from potato tubers, soil, water, and weeds samples were identified by morphological characteristics, physiological, and biochemicals. were identified by PCR and Real-time technique and classified as a Ralstoniasolanacearum race 3 biovar2.Effective ways of creating sustainable agriculture for enhancing crop productiveness with a minimal disturbance to the surroundings is the exploration of plant growth-promoting rhizobacteria and some different microbe-based symbioses in plants.The antagonistic activity of (PGPR) strains used (Bacillus circulans, Paenibacillus polymyxa, Bacillus megatherium, Pseudomonas fluorescence, Azotobacter spp, and Azospirillum brasilense (N040)as single and mixid cultures against the bacterial pathogen (Ralstonia solanacearum) showed that all the tested bioagent exhibited antibacterial effect against Ralstonia solanacearum. The positive response was recorded on the NPK content of plant Shoot and Root as a result of inoculation Ralstonia solanacearum with the biocontrol agents of PGPR strains. The percentage of disease severity was reduced in disease symptoms according to the inoculation of potato tubers with different bioagents (PGPR). The counts of pathogenic bacteria of Ralstonia solanacearum in rhizosphere soil were reduced. In addition, the tested PGPR against R. solanacearum using non-sterilized soil were evaluated for their abilities on reducing potato brown rot or wilt infection caused by R. solanacearum on two potato cultivars (Bellini and Hermes) under greenhouse conditions showed that the individual treating with the biotic treatments before cultivation in non-sterilized inoculated soil with R. solanacearum prevented the appearance of wilt infection for 60 days of cultivation. The ability of the antagonistic bacterial (PGPR) isolates to produce siderophores with R. Solanacearumwas also studies