الفهرس 
REVIEW OF LITERATUREOnly 14 pages are availabe for public view
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Abstract
SUMMARY AND CONCLUSIONS
from results obtained in this research study, the following information could be deduced to create an ideal and perfect protocol for micropropagation of Jacaranda mimosifolia via stem nodes are taken from the tissue culture:
• Select seeds as a primary an explant source for micropropagation.
• Always use freshly-collected seeds from capsules of a certified mother plant.
• Never rely on or use commercially bought seeds as they tend to be less viable (in germination) and also, more than likely they lose their growth vigor because of inappropriate storage conditions.
• Select light brown colored fruit-capsules from the mother plant in October which is a sign of better seeds vigor and higher germination rates than in dark-brown colored fruit capsules.
• Sterilize the freshly-collected seeds in a 10 % solution of common bleach ‘Clorox‘ (5.25 NaOCl) for 9 or 12 min soaking periods. Both soaking periods have proven to be effective to sterilize J. mimosifolia seeds.
• Under sterile conditions, germinate the sterile seeds by placing them in 250 ml glass vessels that contain ¾ strength MS medium + 30 g/l-1 sucrose + 6 g/l-1 agar at pH 5.7.
• After 45 days culture under the laminar flow hood take 1.5-2.0 cm stem nodes after remove of shoot tip.
• For the establishment stage place the excised stem nodes separately every three stem nodes in a 250 ml jar on a hormone-free medium ½ strength MS + 30 g/l-1 sucrose + 8 g/l-1 agar.
• For the multiplication stage, separate well established shoots individually and place every three shoots together in a 250 ml jar on ½ strength MS medium + 30 g/l-1 sucrose + 8 g/l-1 agar. Add 2.0 mg/l-1 6-BAP cytokinin to ensure proliferation as much as possible of shoots. Repeat this process by separating the initiated shoots and culturing them again.
• The process of re-culturing and separating the individual shoots for at is repeated least four times.
• For the rooting stage, place the multiplied shoots individually every three multiplied shoots in a 250 ml glass jar ¼ strength MS medium + 15 g/l-1 sucrose + 8 g/l-1 agar. To improve rooting, add in advance to the culture medium AC (activated charcoal).
• For the acclimatization stage, choose a growing medium that is composed of 2:1 peatmoss: perlite (v/v) which is high in its organic components.
• Transplant the rooted shoots individually in 24 cell foam trays filled beforehand with the growth medium after first dipping their rooted zones in a fungicide solution (0.5 g/l-1 Rhizolex).
• Adopt a dual strategy for the acclimatization stage involving an initial in vitro step for 60 days inside a small plastic covered cabinet inside the lab acclimatization area. This is followed by an ex vitro step inside a greenhouse for another 60 days period.