الفهرس 
Genotypic variance analysis of Cryptosporidium isolates from stool of children in Egypt
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Abstract
Background: Cryptosporidiosis is recognized by WHO as one of child infectious killers and the second cause of diarhoeal diseases and death in infant. Assessing Cryptosporidium genetic diversity is a realistic goal to elucidate its transmission dynamics and design preventive measures in absence of effective treatment.Objectives: A cross sectional study, was designed to determine the genotypic and sub-genotypic prevalence of Cryptosporidium in stool of Egyptian children using 4 diagnostic methods; AF stain coproscopy, copro- nPCR/RFLP, HRM and sequence analysis of Hsp90, Cowp, Gp60. genes A case control study design was implemented to detect the associated risk factors for susceptibility to cryptosporidiosis. Methodology: Stool samples were collected from 335 children complaining of diarrhea and other GIT symptoms (attending outpatient clinic in Abu El Rish hospital, Kasr Al-Ainy School of Medicine, Cairo University, Cairo, Egypt). Their relative data were recorded. Samples were processed for AF stain coproscopy, copro-nPCR/RFLP, HRM and sequence analysis of Hsp90, Cowp, Gp60 genes. Results: Two genotypes C.hominis and C.parvum were identified in 43 isolates from Egyptian children by DNA sequence analysis of COWP and HSP90 genes and by real time PCR high-resolution melting (HRM) assay. Further sub-genotyping phylogenic analysis of DNA sequences at GP60 Abstract locus using PCR-based sequencing identified three C.hominis subtype families (Ib, Id & Ie) and two C. parvum subtype families (IIc & IId)