الفهرس 
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Abstract
This study aimed to detection of some causative agents of fish diseases through:-
- isolation and identification of certain bacterial pathogens”gram negative bacteria” from clinically diseased fish and water at Elmahmoudia , Edfina and marine water and will concentrate on Vibrio spp isolation and identification
This study was carried out on the tilapia fish and water from Nile in El.mahmoudia canal at El-Mahmoudia city and brakish water in Edfina and marine water .
Random sampling was used to select tilapia fish Nile in El.mahmoudia and brakish water in Edfina and marine water . Live fish were transported for necropsy in sterile box
The water samples were collected aseptically using a sterile 500ml screw capped glass bottles . submerged 15cm to 20 cm below the water surface at every sampling,collecting at least 100ml water.
Bottles with water samples were labeled accordingly. Water samples were placed in a cool box packed with ice, then transported to laboratory.
The samples were later transferred freh to laboratory and start microbiological examination.
The results shown that :
(A)In water samples:
The higher incidences of bacteria in water samples observed in Vibrio as its incidences were 16 (80 %) in Elmahmoudia, followed by its incidences in brakish water 4 (66.67 %), and the lower incidences observed in Edfina 13 (65%).
The incidences of Coliformis showed a higher level in El-mahmoudia collected samples 10 (50 %)m followed by Edfina samples 9 (45 %) and samples collected from and in brakish water 1 (16.67 %).
The incidences of Aeromonas showed a higher level in brakish water 3 (50 %), followed by Edfina samples 6 (30 %), followed by samples collected from El-mahmoudia 5 (25 %).
The incidences of Pseudomonas showed a higher level in samples collected from El-mahmoudia 5 (25 %) , followed by Edfina collected samples 4 (20 %) and in brakish water 1 (16.67 %).
The lower incidences of bacteria observed in salmonella as the samples collected from the different localities not recorded any isolates of Salmonellae.
The higher incidences of bacteria observed in the samples collected from Elmahmoudia followed by Edfina and the lower bacterial incidences observed in the samples collected from brakish water.
(B)In fish samples:
The higher incidences of bacteria in fish samples samples observed in Vibrio as its incidences were 18 (90 %) in Elmahmoudia 18 (90 %), followed by its incidences in Edfina 17 (85 %) and the lower incidences observed in brakish water 5 (83.34 %).
The incidences of Coliformis showed a higher level in El-mahmoudia collected samples 16 (80 %) followed by Edfina samples 14 (70 %) and samples collected from and in brakish water 2 (33.30 %).
The incidences of Aeromonas showed a higher level in brakish water 4 (66.67 %), followed by Edfina samples 8 (40 %), followed by samples collected from El-mahmoudia 6 (30 %).
The incidences of Pseudomonas showed a higher level in samples collected collected from brakish water 3 (50 %), followed by samples collected from El-mahmoudia 7 (35 %) and te lower incidences observed in samples collected from Edfina 6 (30 %).
The lower incidences of bacteria observed in Salmonella as its higher incidences observed in the fish samples collected from Edfina 1 (5 %) and in El-mahmoudia 1 (5 %) while, in brakish water fish no incidences recorded in the fish samples.
The higher incidences of bacteria observed in the samples collected from Elmahmoudia followed by Edfina and the lower bacterial incidences observed in the samples collected from brakish fish.
Molecular identification of Vibrio spp. Confirmed V. Para haemolyticus isolated from water and fish sample.
ERIC-PCR show cluster between isolate (fish and related water )and clustering between each samples ranging from 20-100%.In accordance with the current results with other methods using large numbers of V.parahaemolyticus strains.