الفهرس 
Introduction and aim of the workOnly 14 pages are availabe for public view
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Abstract
Endometrial hyperplasia is an abnormal proliferation of the endometrial glands and stroma characterized by the presence of cytological and architectural alterations.
Endometrial carcinoma is one of the most common gynecologic malignancy. The prognosis of this neoplasm is strictly related to the disease stage at diagnosis. Prolonged unrestricted estrogen exposure, age, obesity, diabetes, race, nulliparity, infertility, early age of menarche, family history, late age of menopause, and tamoxifen drug represent important risk factors for development endometrial cancer.
Baculoviral inhibitor of apoptosis protein (IAP) repeat containing 7 (BIRC7), also known as livin, is a member of the IAPs family and was known to have a role in prevention of cell death induced by apoptosis and modulation of cell proliferation and cell cycle. The human BIRC7 is encoded by BIRC7 gene that located on chromosome 20q13.33 and spanned about 4613 bases. The BIRC7 gene consists of seven exons with six introns. Livin overexpression has been reported in several cancer types.
No data were identified concerning the diagnostic/prognostic impact of livin/BIRC7 gene expression on endometrial hyperplasia and carcinoma.
Serum estrogen level was determined by the quantitative competitive enzymelinked immunosorbent assay (ELISA) technique. The serum catalase activity and malondialdehyde level were assessed colorimetrically. In addition, after isolation of RNA from whole blood, reverse transcriptase-polymerase chain reaction (RT-PCR) was done. After amplification, the PCR products were electrophoresed on agarose gel. The products bands were digitally photographed under fixed conditions and analyzed. Livin gene expression was calculated.
we can conclude that livin/BIRC7 gene expression was found to be associated with the endometrial hyperplasia and carcinoma among Egyptian women. Livin/BIRC7 is overexpressed in endometrial carcinoma compared to endometrial hyperplasia. Our findings provide evidence that the detection of livin/BIRC7 may be used as a reliable molecular diagnostic technique for the endometrial carcinoma.