الفهرس 
General Introduction and Literature ReviewOnly 14 pages are availabe for public view
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Abstract
This thesis consists of five parts:
Part I: General Introduction and Literature Review
This part consists of two sections
Section A: General Introduction
It includes a general introduction on large animal respiratory infections including prevalence, etiology, effect on quality of life and treatment options.
Section B: Literature Review
This section includes a detailed review on the studied drugs, Florfenicol (FLR), Flunixin Meglumine (FLU), Oxytetracycline HCl (OXY), Bromhexine HCl (BRH), and Lidocaine HCl (LID). This part also explains chemical and physical drug properties, pharmacology, structure activity relationship, pharmacokinetics and different analytical methods reported in literature.
Part II: Spectrophotometric determination of veterinary antibiotics in their formulations
This part consists of four sections:
Section A: Spectrophotometric determination of Florfenicol and Flunixin Meglumine.
In this section, validated accurate and precise UV spectrophotometric methods have been described here for the simultaneous determination of Florfenicol (FLR) and Flunixin Meglumine (FLU), in their dosage form. The severely overlapped spectra of FLU and FLR were resolved by ratio difference (RD), derivative ratio (DD1), Concentration value (CV), absorbance subtraction (AS), amplitude modulation (AM), and constant multiplication coupled with spectrum subtraction (CM-SS).
The methods were successfully applied for the simultaneous determination of the drugs in synthetic mixtures and Megluflur® injection, their combined dosage form.
Section B: Spectrophotometric determination of Oxytetracycline HCl and Flunixin Meglumine.
In this section, simple and precise UV spectrophotometric methods have been described for the simultaneous determination of Oxytetracycline HCl (OXY) and Flunixin Meglumine (FLU), in their dosage form. The severely overlapped mixture OXY and FLU was determined in this section using constant value via amplitude difference (CV- AD) , constant center method (CC) , advanced concentration value (ACV), ratio subtraction via amplitude difference coupled with spectrum subtraction (RS/AD-SS).
Furthermore, the applied methods were successfully applied for the simultaneous determination of the drugs studied in synthetic mixtures and Floxon® injection, their combined dosage form.
Section C: Spectrophotometric determination of Oxytetracycline HCL, Bromhexine HCl, and Lidocaine HCl
In this section, the ternary mixture composed of OXY, BRH, and LID, was determined by simple and accurate UV spectrophotometric methods, constant multiplication coupled with spectrum subtraction (CM-SS) and successive ratio subtraction coupled with spectrum subtraction (SRS-SS). Successful application of the developed methods in their synthetic mixtures and dosage form, Oxyclear® injection, was also described.
Section D: Comparative study between PLS and PCR in determination of Oxytetracycline HCL, Bromhexine HCl, and Lidocaine HCl
In this last section, the two multivariate methods named partial least square (PLS) and principal component regression (PCR) computed by the MATLAB® software, were conducted. Those methods depend on a large number of variables which lead to extraction of the maximum amount of information required for the determination of the component of interest in the presence of the other. Satisfying accurate and precise results were obtained in synthetic mixtures and veterinary formulation, Oxyclear® injection, for determining OXY, BRH, and LID.
Part III: chromatographic determination of veterinary antibiotics in their formulations
This part consists of two sections:
Section A: Densitometric determination of Oxytetracycline HCL, Bromhexine HCl, and Lidocaine HCl
This section describes a thin layer chromatography approach for the determination of the ternary mixture, OXY, BRH, and LID, and its application in synthetic mixtures and their dosage form Oxyclear® injection. The developing system was methanol: chloroform: acetonitrile: glacial acetic acid(5:2:2:1 v/v/v/v).
Section B: High performance Liquid chromatography determination of Florfenicol and Flunixin Meglumine (1) and Oxytetracycline HCl and Flunixin Meglumine (2) in their formulations and spiked milk samples.
This section proposes a validated rapid and sensitive reversed HPLC method for simultaneous estimation of two binary mixtures FLU with either FLR or OXY, in their pure form and injection dosage forms, and in spiked milk samples. Determination of OXY and FLU mixture was carried out on C18 Kromasil column: (4.6 × 150 Mm. On the other hand, FLR and FLU determination was carried on C18 Intersil column: 4.6 × 250 mm, 5µm. Diode array detection at 220 nm was used for both mixtures.
The methods achieved successful and precise application to synthetic mixtures, Floxon® and Megluflur® veterinary formulation as well as in spiked milk samples.
Part IV: Electrochemical Determination of Veterinary antibiotics in their formulations and spiked samples
This part consists of one section:
Section A: Potentiometric Determination of Flunixin Meglumine and Oxytetracycline HCl
This section proposed two ion selective electrodes, conventional and CNT-GCE electrodes, for determination of FLU potentiometrically. The method was successfully applied to the dosage form and to spiked milk samples.
This section also proposed a CNT-GCE ion selective electrode for the determination of OXY in addition to another CNT-GCE-(β-CD) modified electrode. The two electrodes were compared successfully applied to the dosage form and to spiked milk samples.
Part V: General Discussion:
It includes a comparison between the different proposed methods and highlight the advantages offered by each method.
This thesis consists of 60 figures, 70 tables, 290 references and ends with an Arabic summary.