الفهرس 
Antitumor and antioxidant activities of solandra grandiflora and cassia alata
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Abstract
This study aims to investigate the major constituents of Solandra grandiflora Sw. and Cassia alata (L.) Roxb. leaves. In addition, to evalute the antitumor and antioxidant activity of the crude methanol extract and its fractions. Phytochemical screening of the crude methanolic extract, determination of total phenolics and total flavonoids of S. grandiflora and C. alata leaves were investigated. Hexane fraction of each plant was saponified into unsap. and sap. (Fatty acids), then analyzed by GC/MS. Fatty acids composition of S. grandiflora leaves comprised a much higher proportion of linolenic, palmitic, linoleic and stearic acids. C. alata leaves were found to comprise a much higher proportion of palmitic, Ü-linolenic, linoleic and stearic acids. Crude fractions of each plant was fractionated by silica gel and sephadex LH-20 column chromatography and further purified by preparative HPTLC and paper chromatography. Each separated pure compound was identified by 1H-NMR, Mass spectrum and UV. Emodin, aloe-emodin, rhein, kaempferol-3-O-gentiobioside, aloe-emodin-8-O-Ý-D-glucopyranoside, kaempferol, quercetin, rhein-8-O-Ý-D-glucopyranoside and emodin-8-O-Ý-D-glucopyranoside were isolated and identified from C. alata leaves. In addition, scopoletin and aesculin were isolated and identified from S. grandiflora leaves. The methanolic extract and its fractions of S. grandiflora and C. alata leaves were tested as anti-proleferation on HepG2 (hepatocellular carcinoma cells), Caco2 (colon adenocarcinoma cells) and MDA-MB-231 (triple negative breast cancer cells). Both plants exhibited cytotoxic activity against tested cells especially chloroform fraction of S. grandiflora with IC₅₀ 11.1 and 16 æg/ml for HepG2 and Caco2 cells, respectively. In addition, the antioxidant activity was estimated and the ethyl acetate fraction of C. alata only showed strong DPPH scavenging activity with EC₅₀ 22.1 æg/ml