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العنوان
The potential role of cyclophosphamide–biopolymeric based composites against nephrotoxicity in rats \
المؤلف
khalifa, Ibrahim Ahmed El Desoky Ibrahim.
هيئة الاعداد
باحث / إبراهيم أحمد الدسوقي ابراهيم خليفة
مشرف / ايمان محمد صالح
مشرف / عاطف سمير درويش
مشرف / رشا الشريف حسن
تاريخ النشر
2022.
عدد الصفحات
144 P. :
اللغة
الإنجليزية
الدرجة
ماجستير
التخصص
Biochemistry
تاريخ الإجازة
1/1/2022
مكان الإجازة
جامعة عين شمس - كلية العلوم - الكيمياء الحيوية
الفهرس
Only 14 pages are availabe for public view

from 144

from 144

Abstract

Cyclophosphamide (CP) is an effective anti-cancer alkylating agent, while it also possesses a wide spectrum of cytotoxicity to normal cells such as nephrotoxicity. Nanoparticles are used as drug carriers and imaging to increase drug solubility, stability and control drug release and specific transport and reduce drug toxicity. Colon Cancer remains one of the major worldwide causes of cancer related morbidity and mortality in both genders. The current study was constructed to evaluate the ameliorating effect of the newly synthesized CP-Egg albumin /carbon nanotube nanocomposite (CP-EA/ CNT) against CP induced nephrotoxicity in rats and to investigate the effectiveness of the newly synthesized composite in treating cancer. To achieve this goal In vivo study was carried out. The physicochemical properties of the newly synthesized composite were also assessed by characterization techniques such as TEM, SEM, zeta-potential, FT-IR and XRD. Preliminary experiment were carried out on 6 groups (10 rats in each group) to calculate LD50 for newly synthesized composite (CP-EA/CNT). In vivo, A) 24 adult male rats were randomly divided into three equal groups (8 rats/group), (1) control group, (2) CP group, (3) CP-EA/CNT group. B) Another Pilot study on colon cancer model was carried out wherever another 32 adult male rats were divided into 4 groups (8 rats /group), (1)control group, (2)DMH group, (3) (DMH+CP) group, (4) (DMH+CP-EA/CNT) group. After experimental period, serum renal biomarkers (creatinine, urea, uric acid, BTP, nepherin, interleukin 8(IL8) and NGAL were evaluated. In addition, histopathological examination of kidney and colon was performed. CP caused nephrotoxicity includes increase in (creatinine, urea, uric acid, BTP, nephrin, IL8 and NGAL) levels for groups that were injected with CP only in both (experiment with healthy rats and pilot study with rats had colon cancer). Groups were injected with CP-EA/CNT nanocomposite revealed a significant improvement in renel biomarkers levels and also improve in Histopathological disorders of kidney and colon. Ultimately, the outcomes of this study ascertained the ameliorating role of the newly synthesized CP-EA/CNT nanocomposite against nephrotoxicity induced by CP in rats without interfering with CP anticancer properties.