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العنوان
Utilization of Some Cyanobacteria in Biological Control against Fusarium Oxysporum f. sp. Lycopersici /
الناشر
Reham Salah Abd El-wahab Mohamed Hasan,
المؤلف
Hasan, Reham Salah Abd El-wahab Mohamed,
هيئة الاعداد
باحث / Reham Salah Abd El-wahab Mohamed Hasan
مشرف / Mohamed Ali Deyab
مشرف / Mostafa Mohamed El-Sheekh,
مشرف / Yossef Elsadany
مشرف / Seham El-Sayed Abu Ahmed
الموضوع
Biological Control. Cyanobacteria. Nostoc calcicola. Spirulina platensis.
تاريخ النشر
2021.
عدد الصفحات
113 p. :
اللغة
الإنجليزية
الدرجة
الدكتوراه
التخصص
علوم النبات
الناشر
Reham Salah Abd El-wahab Mohamed Hasan,
تاريخ الإجازة
1/1/2021
مكان الإجازة
جامعة دمياط - كلية العلوم - النبات والميكروبيولوجى
الفهرس
Only 14 pages are availabe for public view

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from 141

Abstract

Soil samples were collected from 8 different sites in El-Hamoul and Sidi Salem centers in Kafr El-Sheikh governorate, from the surface layer of the soil with estimation of the chemical and physical properties of the soil samples, which showed diversity in soil components, salinity and pH, and this difference led to the diversity of the existing cyanobacterial isolates.
Three cyanobacterial strains were isolated and purified from the soil and identified using their morphology and physiological activity. Nostoc calcicola, Nostoc linkia, and Anabaena variabilis were identified. Spirulina platensis was also obtained from the microbiological laboratory to put it under study and compare with other isolates isolated from soil Using GC-MS technology, forty nine bioactive compounds were identified in the methanol extract of Nostoc calcicola. Thirty-five biologically active compounds were identified in the methanol extract of Nostoc linkia. Thirty seven bioactive compounds were identified in Anabaena variabilis extract.
The lowest content of bioactive compounds (7 compounds) was determined in the methanolic extract of Spirulina platensis
The study proved that the soil algae (Nostoc calcicola - Anabaena variabilis - Spirulina platensis) gives a high productivity (9-7 times) in terms of the number of active natural compounds than those extracted from the aquatic algae that was grown in the laboratory, which confirms the impact of the environment on the level of productivity Algae for quantitative and qualitative active substances and all of these extracted active compounds have a scientific reference in their use in agricultural, medical, food and industrial fields.
The role of pollination with some cyanobacteria such as Nostoc calcicola, N. linkia, Anabaena variabilis, Spirulina platensis on root, plant height, number of fruits and weight of tomato fruits was studied. The experiment was divided into two phases after 50 and 100 days. The difference in the effect on tomato plant growth was observed according to the type of algae and the type of extract.
from these results, it became clear to us that Fusarium gives clear vegetative growth to the tomato plant in terms of plant height at the beginning of plant growth and then the plant begins to gradually die as a result of drying out the roots and then drying the whole plant, including the fruits and leaves.
After 100 days, the results showed that the highest yield of tomato fruits in terms of number and weight was for tomato plants treated with: N. linkia (culture), (extract) compared to plants not treated with cyanobacteria (without addition) (control 1), as well as in the case of plants Infected with Fusarium only (Control 2).
The study also showed that there is a noticeable discrepancy in the growth of tomato fruits according to the type of algae and the method of use (algal culture, algal extract or the combination of them).
Thus, the results showed the diversity of cyanobacteria in agricultural soil and the possibility of using the culture, methanolic extracts, or a combination of them for these cyanobacteria to improve the growth of tomato plants at the vegetative and fruit levels, in addition to the biological resistance to Fusarium and protecting the tomato plant from its damages depending on the diverse content of cyanobacteria on the compounds biologically active ingredients that exceed 49 biologically active compounds.