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العنوان
Physiological and molecular studies associated with engineering dehydration- stress tolerance in sesame /
الناشر
Amal Farouk Abdelhamied Alshafeay ,
المؤلف
Amal Farouk Abdelhamied Alshafeay
هيئة الاعداد
باحث / Amal Farouk Abdelhamied Alshafeay
مشرف / Mohamed Ramadan Aboulella Nesie
مشرف / Ahmed Shawky Ibrahim
مشرف / Mohamed Saleh Tawfik
تاريخ النشر
2018
عدد الصفحات
120 P. :
اللغة
الإنجليزية
الدرجة
الدكتوراه
التخصص
علوم النبات
تاريخ الإجازة
13/7/2019
مكان الإجازة
جامعة القاهرة - كلية الزراعة - Agricultural Botany
الفهرس
Only 14 pages are availabe for public view

from 158

from 158

Abstract

Drought is a major stress that globally restricting the agricultural expansion and crops productivity. Sesame (Sesamum indicum L.) considered as a recalcitrant plant to be regenerated. In this study for improving the dehydration stress tolerance of sesame plant, a LOS5 of Arabidopsis thaliana was introduced to de-embryonated cotyledons of sesame cv. Sohag1 via Agrobacterium tumefaciens strain LBA4404 which harboring the LOS5 gene encode a molybdenum cofactor sulfurase (MCSU), which catalyzes the generation of the sulfurylated form of MoCo, a cofactor required by aldehyde oxidase to functions in the last step of ABA biosynthesis, leading to ABA accumulation in tissues followed by enhancing the drought stress tolerance. The insertion of AtLOS5 was confirmed by PCR and RT-PCR analysis