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العنوان
Selection for Resistance to Nematode in Some Genotypes of Tomato /
الناشر
Manal Mohamed El-Sayed Zaater,
المؤلف
Zaater, Manal Mohamed El-Sayed.
هيئة الاعداد
باحث / Manal Mohamed El-Sayed Zaater
مشرف / Mohamed Saad Ibrahim Hamada
مشرف / Mohamed Hassan Abd El-Aziz Moussa
الموضوع
النبات، علم. Tomato.
تاريخ النشر
2020.
عدد الصفحات
179 p. :
اللغة
الإنجليزية
الدرجة
الدكتوراه
التخصص
الهندسة الزراعية وعلوم المحاصيل
تاريخ الإجازة
29/12/2020
مكان الإجازة
جامعة دمياط - كلية الزراعة - الوراثة
الفهرس
Only 14 pages are availabe for public view

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Abstract

Objectives:
Study the genetic diversity between the Tomato genotypes used in this study using molecular marker
techniques such as SCoT technique. Also, studying the effect of nematode infections on plant growth traits,
study the host suitability of Tomato genotypes to (M. incognita) infections under greenhouse conditions.
In addition to estimate the biochemical parameters for uninfected and infected Tomato genotypes with nematode under greenhouse conditions.
Methods & Results:
At the spring season of 2019 the seeds of the studied genotypes were sown in multi-well foam trays filled with fertilized peat moss. After the appearance of the leaves, the fresh young leaves were collected from each genotype to extract DNA in order to study the variation between all genotypes ”4 parents, their 12 hybrids” and check variety, SCoT technique has been used.
Then the parents and their hybrids were evaluated under conditions of both infection and non-infection of a Root-Knot Nematode in the summer season of (2019) at a private net house in Kafr Saad, Damietta under the supervision of the Dept. of Genetics, Fac. of Agric., Damietta University.
The obtained results could be summarized as follows:
1-The nine SCoT primers used through Polymerase Chain Reaction succeeded in targeting the amplification of many DNA sequences of the genotypes of tomatoes used in this study. Results of the products of this interaction indicated the effectiveness of SCoT in showing the molecular variation between the genotypes used.
2 -The results of the effect of nematode (M. incognita) infections on plant growth traits under greenhouse conditions indicated that (P4) ‟Castle Rock were severely affected by Nematode infection. Whereas it accomplished the highest reduction values. On the other side, the hybrid (P2 x P4) accomplished the lowest reduction values
3- The results of studying the host suitability of Tomato genotypes to (M. incognita) infections under greenhouse conditions indicated that none of the tested genotypes was immune to root-knot nematode, M. incognita, though the incidence varied from genotype to another, while only the genotype (P2 x P4) was found to be resistant, the genotypes (p1) and (P4 x P3) were found to be moderately resistant.
Conclusion:
from studying the genetic diversity using SCoT technique, doing the artificial infection and analyze the data of complete diallel analysis, it could be concluded that, the molecular marker (SCoT) exhibited robust amplification profiles. So, it can be used to differentiate the genotypes and studying the genetic diversity between these studied genotypes. Of the screened genotypes against nematode, the genotype (P2 x P4) exhibited the lowest rate of nematode reproduction (RF=0.12). from the diallel analysis, the results revealed that mean squares of the studied genotypes were significant for all studied traits and parameters, which proves the presence of a considerable amount of genetic variation among studied genotypes. Also, GCA and SCA mean squares were highly significant for all studied traits. This means that both GCA and SCA were important in the inheritance of these traits. Likewise, reciprocal effect mean squares were found significant for all the studied traits indicating that these traits are controlled by extra-nuclear factors in addition to the nuclear factors. Also, data showed that significant SCA effects in demand direction for most crosses in some studied traits. The estimation of genetic parameters and heritability for all studied traits proved the predominance of non-additive gene action in the inheritance of these traits. So, from all the previous results we can conclude that, the cross (Cherry x Castle Rock) is a superior hybrid, and it could be selected in the breeding programs to resist nematode. Therefore, using this combination as started material for selection breeding program to produce resistant root-knot nematodes genotypes.